中草药2026，Vol.57Issue(10)：3720-3729,10.DOI:10.7501/j.issn.0253-2670.2026.10.005
黄芩-炙甘草配伍合煎液不同相态的体外释放及药动学研究
Study on in vitro release and pharmacokinetic of different phases of co-decoction of Scutellariae Radix and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle
摘要
Abstract
Objective To investigate the compositional distribution,in vitro release,and pharmacokinetic behavior of different phases in the Huangqin(Scutellariae Radix,SR)-Zhigancao(Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,GRRPM)decoction,and to elucidate the physical structural basis for the delayed and enhanced efficacy mediated by herb compatibility.Methods The decoction was separated into nanoparticulate,true solution,and precipitate phases by centrifugation combined with dialysis.The particle size and morphology of these phases were characterized.The contents of major active components baicalin,wogonoside,liquiritin apioside,glycyrrhizic acid,and others were determined by HPLC,and their in vitro release profiles were evaluated.The plasma concentration of baicalin in rats after oral administration of different phases was measured using LC-MS/MS,and pharmacokinetic parameters were calculated.Results Significant differences in particle size and morphology were observed among the phases.The mass fractions of liquiritin apioside,baicalin,wogonoside,and glycyrrhizic acid in each phase decreased in the order:nanoparticulate phase>true solution phase>precipitate phase.All four components showed the highest release from the nanoparticulate phase,followed by the true solution phase,and the lowest release from the precipitate phase.After oral administration of the whole extract and the different phases,the Cmax and AUC0—t of baicalin ranked as follows:nanoparticulate phase>whole extract>true solution phase>precipitate phase.Conclusion The formation of a complex multiphase system in the SR-GRRPM decoction significantly affects the spatial distribution,in vitro release,and pharmacokinetic behavior of the active constituents.This may represent the physical structural basis underlying the delayed and enhanced efficacy resulting from the combination of the two herbs.关键词
配伍/黄芩/炙甘草/相态差异/成分分布/体外释放/药动学Key words
compatibility/Scutellariae Radix/Glycyrrhizae Radix et Rhizoma Praeparata cum Melle/phase difference/component distribution/in vitro release/pharmacokinetics分类
医药卫生引用本文复制引用
张双辰,何沂灿,张年战,陈芳雯,魏欣玲,沈成英,岳鹏飞,申宝德..黄芩-炙甘草配伍合煎液不同相态的体外释放及药动学研究[J].中草药,2026,57(10):3720-3729,10.基金项目
国家自然科学基金资助项目(82460852) （82460852）
江西省自然科学基金资助项目(20232BAB216139) （20232BAB216139）
江西中医药大学校级科技创新团队发展计划项目(CXTD22006) （CXTD22006）
