针刺研究2026，Vol.51Issue(5)：602-613,12.DOI:10.13702/j.1000-0607.20251131
"内膝眼"及"外膝眼"仿灸干预膝关节骨关节炎大鼠优效温度-波长参数研究
Study on optimal temperature-wavelength parameters of moxibustion simulator intervention at"Neixiyan"(EX-LE4)and"Waixiyan"(ST35)acupoints for knee osteoarthritis in rats
摘要
Abstract
Objective To identify the optimal temperature and infrared radiation wavelength parameters of moxibustion simulator intervention at"Neixiyan"(EX-LE4)and"Waixiyan"(ST35)acupoints for ameliorating knee osteoarthritis(KOA)in rats.Methods Male Sprague-Dawley(SD)rats were randomly assigned to a normal group,a model group,EX-LE4 or ST35 mild moxibustion groups,and EX-LE4 or ST35 moxibustion simulator groups with different wavelengths,with 6 rats in each group.The KOA model was established by intra-articular injection of a mixed solution of L-cysteine and papain into the left knee joint cavity.An infrared radiation measurement device was used to collect the characteristic infrared radiation wavelengths of EX-LE4 and ST35 acupoints.The mild moxibustion group received mild moxibustion intervention at EX-LE4 and ST35 acupoints separately with a moxa stick at 43℃,while the moxibustion simulator groups were treated with photothermal simulated stimulation with a fixed temperature of 43℃combined with different wavelengths(1 652,3 340,and 7 500 nm).The spatial,temporal and dynamic parameters of rat gait were analyzed.ELISA was performed to quantitatively determine the serum content of interleukin-10(IL-10),an anti-inflammatory cytokine.HE staining and Masson staining were conducted to evaluate the inflammatory response and collagenization degree of synovial tissue of the knee joints.Results The characteristic infrared radiation wavelength of the acupoints was approximately 3 340 nm in rats under the pathological state of KOA.Compared with the normal group,the model group showed significant alterations in gait spatial,temporal and dynamic parameters(P<0.001),a marked decrease in serum IL-10 level(P<0.05),as well as significant elevations in synovial Krenn score and collagen area percentage(P<0.000 1).In comparison with the model group,the mild moxibustion group and moxibustion simulator groups at both EX-LE4 and ST35 acupoints exhibited varying degrees of improvement in gait spatial parameters(P<0.001,P<0.01,P<0.05)and dynamic parameters(P<0.001,P<0.01).The mild moxibustion group,1 652 nm moxibustion simulator group and 3 340 nm moxibustion simulator group at both acupoints also achieved improvements in gait temporal parameters(P<0.01,P<0.05,P<0.001),with the mild moxibustion group and 3 340 nm moxibustion simulator group showing superior ameliorative effects compared with the moxibustion simulator groups with other parameters(P<0.01,P<0.001,P<0.05).Compared with the model group,the EX-LE4 mild moxibustion group,EX-LE4 3 340 nm moxibustion simulator group and ST35 3 340 nm moxibustion simulator group presented a significant increase in serum IL-10 contents(P<0.05,P<0.000 1,P<0.001).Moreover,the serum IL-10 contents in the EX-LE4 3 340 nm moxibustion simulator group was significantly higher than those in the moxibustion simulator groups with other parameters(P<0.000 1,P<0.01).In contrast to the model group,the mild moxibustion groups and 3 340 nm moxibustion simulator groups at both acupoints displayed significant reductions in synovial Krenn score and collagen area percentage(P<0.01,P<0.000 1,P<0.05,P<0.001),with the 3 340 nm moxibustion simulator group yielding the best therapeutic outcome(P<0.05).Conclusion Moxibustion intervention with the parameter combination of 43℃and 3 340 nm at EX-LE4 and ST35 acupoints can significantly improve gait function and suppress inflammatory response in KOA rats,with consistent optimal parameter combinations for both acupoints.The synergistic effect may be attributed to the acupoint-moxibustion infrared radiation resonance effect formed between the specific wavelength infrared radiation generated by moxibustion and the spontaneous infrared radiation emitted by the acupoints of rats under KOA pathological conditions.关键词
仿灸/膝关节骨关节炎/灸温/红外辐射波长/共振效应Key words
Moxibustion simulator intervention/Knee osteoarthritis/Moxibustion temperature/Infrared radiation wavelength/Resonance effect引用本文复制引用
郭亚婷,高俊虹,刘群,辛娟娟,赵玉雪,闫文茜,陆嘉盈,江天云.."内膝眼"及"外膝眼"仿灸干预膝关节骨关节炎大鼠优效温度-波长参数研究[J].针刺研究,2026,51(5):602-613,12.基金项目
国家自然科学基金项目(No.82305405) （No.82305405）
中央级公益性科研院所基本科研业务费专项资金项目(No.ZZ16-XRZ-076、ZZ17-YQ-028、ZZ-YC2023004) （No.ZZ16-XRZ-076、ZZ17-YQ-028、ZZ-YC2023004）
