中国病理生理杂志2026，Vol.42Issue(5)：946-953,8.DOI:10.3969/j.issn.1000-4718.2026.05.012
蒺藜皂苷通过抑制STAT3磷酸化减少蛛网膜下腔出血大鼠脑组织促炎细胞因子表达和神经元凋亡
Tribuloside attenuates neuronal apoptosis and pro-inflammatory cyto-kine release after subarachnoid hemorrhage in rats by inhibiting STAT3 phosphorylation
摘要
Abstract
AIM:This study aims to investigate whether tribuloside attenuates neuronal apoptosis and inflam-matory response in the basal cortex of rats with subarachnoid hemorrhage(SAH)via modulation of the signal transducer and activator of transcription 3(STAT3)pathway,thereby providing experimental evidence for potential therapeutic strate-gies for SAH treatment.METHODS:A rat SAH model was established using endovascular perforation.Animals were as-signed to a sham-operated group and SAH model groups evaluated at 6,12,24,48 and 72 h.The levels of STAT3 and phosphorylated STAT3(p-STAT3)in the basal cortex were assessed by Western blot.Molecular docking was performed to evaluate the binding affinity between tribuloside and STAT3,and to identify potential binding sites.Tribuloside was ad-ministered intraperitoneally,and the rats were divided into sham group,SAH 48 h group,and low-,medium-and high-dose tribuloside groups.The protein levels of STAT3,p-STAT3,apoptosis-related proteins[B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),caspase-8,caspase-9 and caspase-3],and inflammatory factors[interleukin-4(IL-4),IL-10,IL-6 and IL-1β]were detected by Western blot.Neuronal apoptosis was quantified using TUNEL staining,and the levels of cytokines[tumor necrosis factor-α(TNF-α),IL-6,IL-4,IL-1β,IL-18 and IL-10]were measured by ELISA.RESULTS:The phosphorylation of STAT3 was significantly increased in SAH groups at 6 to 72 h compared with sham group.Molecular docking demonstrated direct binding between tribuloside and STAT3.Compared with model group,tribuloside treatment significantly reduced STAT3 phosphorylation,down-regulated pro-apoptotic proteins(Bax,caspase-8,caspase-9 and caspase-3),up-regulated the anti-apoptotic protein Bcl-2,decreased the number of TUNEL-positive cells and the levels of pro-inflammatory factors(TNF-α,IL-6,IL-1β and IL-18),and elevated the levels of anti-inflammatory cytokines(IL-4 and IL-10).CONCLUSION:Tribuloside effectively alleviates neuroinflammation and neu-ronal apoptosis in the basal cortex of SAH rats,potentially through direct modulation of the STAT3 signaling pathway.关键词
蒺藜皂苷/蛛网膜下腔出血/信号转导及转录激活因子3/神经元凋亡/细胞因子Key words
tribuloside/subarachnoid hemorrhage/signal transducer and activator of transcription 3/neuro-nal apoptosis/cytokine分类
医药卫生引用本文复制引用
侯亚军,郭宁,张琬晶,赵佳,王玮琦,王世军..蒺藜皂苷通过抑制STAT3磷酸化减少蛛网膜下腔出血大鼠脑组织促炎细胞因子表达和神经元凋亡[J].中国病理生理杂志,2026,42(5):946-953,8.基金项目
山东省自然科学基金资助项目(No.ZR2022QH169) （No.ZR2022QH169）
泰安市科技创新发展项目(No.2024NS141) （No.2024NS141）
山东省中医药科技项目(No.M20253702) （No.M20253702）
