中国草食动物科学2026，Vol.46Issue(3)：38-47,10.DOI:10.3969/j.issn.2095-3887.2025.00.071
黄牛源A型产气荚膜梭菌生物学特性及全基因组测序分析
Biological Characterization and Whole-Genome Sequencing Analysis of Clostridium perfringens Type A from Bovine
摘要
Abstract
To determine the causative agent of sudden death cases in cattle at a large-scale farm in Hezhou city,Guangxi,tissue samples from the intestine,lung,and spleen were aseptically collected during necropsy.A comprehen-sive analysis was performed,including bacterial isolation and identification,biochemical testing,growth curve draw-ing,antibiotic susceptibility testing,pathogenicity assessment,and whole-genome sequencing.The result showed that through isolation and purification,the bacterium was identified as Clostridium perfringens type A based on its bio-chemical profile and the presence of type-specific virulence genes.The isolate was designated strain HZ25-1.Antibio-tic susceptibility testing demonstrated that the strain was resistant to nine commonly used antimicrobials,including ceft-iofur,cefalexin,cefoxitin,ect.,while remaining susceptible to four antibiotics,such as penicillin,amoxicillin,ampicil-lin and ofloxacin.Pathogenicity experiments confirmed that all challenged mice died within 24 hours,and the identical bacterium was recovered from the lesions,which confirmed its pathogenicity.The whole genome sequencing analysis revealed that the HZ25-1 strain has a genome length of 3 335 730 bp,with a GC content of 28.12％.The genome was found to be structured with 3 003 coding sequences(CDSs),67 tRNAs,66 ncRNAs,129 repetitive sequences,and 4 ge-nomic islands.Based on integrated annotations from the GO,KEGG,and CAZy databases,it was demonstrated that growth of Clostridium perfringens is primarily supported by the enzymatic hydrolysis of starch and sucrose.Annotation against the VFDB database indicated that the pathogenicity of HZ25-1 was likely enhanced by the synergistic action of the plc,cpb2,and pfoA virulence genes.Furthermore,when annotated using the CARD database,the ermQ and rpoB genes were identified as key determinants conferring the multidrug-resistant phenotype observed in the HZ25-1 strain.Phylogenetic analysis of the 16S rRNA gene showed HZ25-1 was most closely related to a Greek buffalo strain(JX311438.1),and clusters with strains from Indian cattle(KF999873.1),Shandong deer(OK175676.1),Yunnan goats(MK156683.1),and Qinghai yaks(MW980103.1),suggesting potential cross-regional and cross-host transmission.关键词
产气荚膜梭菌/生物学特性/致病性/耐药性/全基因组测序Key words
Clostridium perfringens/biological characteristics/pathogenicity/antimicrobial resistance/whole-genome sequencing分类
农业科技引用本文复制引用
刘赛安,彭昊,雷磊,钟舒红,吴翠兰,李常挺,李慧慧,王乐平,马春霞,白慧丽,丁嘉烽..黄牛源A型产气荚膜梭菌生物学特性及全基因组测序分析[J].中国草食动物科学,2026,46(3):38-47,10.基金项目
广西重点研发计划(桂农科AB241484045) （桂农科AB241484045）
国家现代产业体系广西肉牛肉羊创新体系建设项目(nycytxgxcxtd20210905) （nycytxgxcxtd20210905）
来宾市重点研发计划(来科产241516) （来科产241516）
桂科专项(24-2) （24-2）
广西农业农村厅自筹科技项目(Z2023031) （Z2023031）
