中国防痨杂志2026，Vol.48Issue(6)：787-794,8.DOI:10.19982/j.issn.1000-6621.20260021
民族药阴地蕨抗结核活性筛选及化学成分分析
Screening of anti-tuberculosis activity and analysis of chemical constituents of the Ethnomedicine Botrychium ternatum
摘要
Abstract
Objective:To screen ethnomedicines with anti-tuberculosis activity,evaluate their efficacy and chemical composition,and provide new insights for tuberculosis treatment.Methods:Fifteen ethnomedicines were selected through literature review,and their total extracts were prepared.Initial screening for activity against Mycobacterium smegmatis(M.sm)was performed using the solid agar disk diffusion method.The medicine with the best activity was further extracted to obtain organic solvent fractions(petroleum ether,dichloromethane,n-butanol).The minimum inhibitory concentrations(MIC)of these fractions against M.sm and Bacillus Calmette-Guérin(BCG)were determined by the resazurin microtiter broth dilution assay.The activity against the standard strain H37Rv and clinical isolates(including drug-resistant and drug-sensitive strains)of Mycobacterium tuberculosis was evaluated via the microbroth dilution method.The chemical constituents of the total extract and petroleum ether fraction from Botrychium ternatum were analyzed by high-performance liquid chromatography-mass spectrometry(HPLC-MS)to preliminarily explore the potential anti-tuberculosis components.Results:Among the 15 ethnomedicines screened,10 exhibited inhibitory activity against M.sm,with Botrychium ternatum showing the strongest activity(inhibition zone diameter:18.33±4.04 mm).Resazurin assay results indicated significant inhibitory effects for the total extract and the petroleum ether fraction of Botrychium ternatum against M.sm and BCG,with MICs of 1875 μg/ml against M.sm for both,and MIC of 2 μg/ml,and 1 μg/ml against BCG for total extract and the petroleum ether fraction,respectively.Microbroth dilution testing against 11 clinical isolates of Mycobacterium tuberculosis showed that the MICs for the total extract and the petroleum ether fraction mainly ranged from 14 to 58 μg/ml,with the lowest MIC for the petroleum ether fraction reaching 14 μg/ml.HPLC-MS analysis identified 44 compounds in the total extract and 33 compounds in the petroleum ether fraction of Botrychium ternatum.Conclusion:Botrychium ternatum demonstrated the best activity against M.sm among the 15 ethnomedicines.Further evaluation confirmed its significant anti-tuberculosis activity,particularly for its petroleum ether fraction.This study not only verified the feasibility of screening anti-tuberculosis agents from ethnomedicines,but also provided important candidate substances and scientific basis for developing new anti-tuberculosis drugs through compositional analysis.关键词
分枝杆菌,结核/药物活性筛查/最低抑菌浓度/民族药/成分分析/阴地蕨Key words
Mycobacterium tuberculosis/Drug screening assays/Minimum inhibitory concentration/Ethnomedicines/Compositional analysis/Botrychium ternatum分类
医药卫生引用本文复制引用
李静,周培富,代小伟,陈双双,陈春华,杨国祥,杨承闹,郑禄云,谭承建,李传友,李洁..民族药阴地蕨抗结核活性筛选及化学成分分析[J].中国防痨杂志,2026,48(6):787-794,8.基金项目
国家自然科学基金项目(82260404) （82260404）
贵州省基础研究计划项目(自然科学)面上项目(黔科合基础MS[2025]221) National Natural Science Foundation of China(82260404) （自然科学）
General Program of Natural Science,Basic Research Program of Guizhou Province(Qian Ke He Jichu-MS[2025]221) （Qian Ke He Jichu-MS[2025]221）
