局解手术学杂志2026，Vol.35Issue(5)：414-420,7.DOI:10.11659/jjssx.03E025020
NDC80对宫颈癌细胞增殖、迁移和侵袭的影响机制研究
Study on the mechanism of NDC80 in regulating proliferation,migration,and invasion of cervical cancer cells
摘要
Abstract
Objective To investigate the expression of NDC80 in cervical cancer and its mechanism in regulating the proliferation,migration,and invasion of cervical cancer cells.Methods The NDC80 expression in cervical cancer and other multiple tumors was analyzed using the GEPIA database.Cancer tissues were collected from 80 cervical cancer patients who underwent surgical resection at the Third Hospital of Shanxi Medical University,along with normal cervical tissues from 80 patients with uterine leiomyoma who underwent surgical resection.The expression levels of NDC80 protein were detected by immunohistochemistry.The expression of NDC80 mRNA in normal cervical cells and cervical cancer cells was detected by qRT-PCR.NDC80 small interfering RNA(NDC80-siRNA)and its negative control(si-NC)were respectively transfected into cervical cancer cell lines.The CCK-8 assay was used to detect the proliferation ability of cervical cancer cells,the wound healing assay was used to detect the migration ability of cervical cancer cells,the Transwell assay was used to detect the invasion ability of cervical cancer cells,and Western blot was used to detect the expression levels of p-PI3K and p-Akt proteins in cervical cancer cells.Results GEPIA database analysis showed that NDC80 was highly expressed in various types of tumors,including cervical cancer,bladder urothelial carcinoma,breast cancer,head and neck cancer,and lung cancer(P<0.05).The cervical cancer tissues showed significantly higher expression level of NDC80 than that in normal cervical tissues(P<0.05).The cervical cancer cell lines SiHa,CaSki,HeLa,and C-33A showed higher expression of NDC80 than that in normal cervical epithelial cells,with the most significant expression observed in SiHa and CaSki cells(P<0.05).Knockdown of NDC80 significantly inhibited the proliferation,migration,and invasion of cervical cancer cells,and also suppressed the expression levels of p-PI3K and p-Akt proteins(P<0.05).Conclusion NDC80 is highly expressed in cervical cancer tissues and may promote the proliferation,migration and invasion of cervical cancer cells.This effect may be mediated by targeting the PI3K/Akt signaling pathway.关键词
核分裂周期蛋白80/宫颈癌/增殖/迁移/侵袭/PI3K/Akt信号通路Key words
nuclear division cycle 80/cervical cancer/proliferation/migration/invasion/PI3K/Akt signaling pathway分类
医药卫生引用本文复制引用
郑佳慧,龚秀芳,花震丹,褚莹,杨柳,廖冀贤,王赞宏..NDC80对宫颈癌细胞增殖、迁移和侵袭的影响机制研究[J].局解手术学杂志,2026,35(5):414-420,7.基金项目
中央引导地方科技发展资金(YDZJSX2022B012) （YDZJSX2022B012）
