中国临床药理学杂志2026，Vol.42Issue(7)：954-961,8.DOI:10.13699/j.cnki.1001-6821.2026.07.009
汉黄芩素通过LncRNA SNHG16/miR-302a-3p/E2F1分子轴调控胃癌细胞生物学行为研究
Research of baicalin regulates the biological behavior of gastric cancer cells through the LncRNA SNHG16/miR-302a-3p/E2F1 molecular axis
摘要
Abstract
Objective To explore the effects of woginostatin(WOG)on the biological behaviors of gastric cancer cells through long non-coding RNA small nucleolar RNA host gene 16(LncRNA SNHG16)/microRNA(miR)-302a-3p/E2F transcription factor 1(E2F1).Methods In cell experiment,human gastric cancer cells(MGC-803)were divided into cell control group(normal culture),cell experimental-H group(100 pmol·L-1 WOG treated cells),oe-NC group(transfected oe-NC),oe-LncSNHG16 group(transfected oe-LncSNHG16),mimic-NC group(transfected mimic-NC),miR-302a-3p mimic group(transfected miR-302a-3p mimic),WOG+oe-NC group(transfected oe-NC based on 100 μmol·L-1 WOG treated cells),WOG+oe-LncSNHG16 group(transfected oe-LncSNHG16 based on 100 μmol·L-1 WOG treated cells),WOG+oe-LncSNHG16+miR-302a-3p mimic group(transfection of oe-LncSNHG16 and miR-302 a-3p mimic on the basis of 100 μmol·L-1 WOG treatment group).In animal experiment,the subcutaneous transplanted tumor model of gastric cancer was established in BALB/C male nude mice.The nude mice were randomly divided into animal experimental-L,-M and-H dose groups(15,30 and 60 mg·kg-1 WOG gavage),and the animal control group was gavaged with equal volume of physiological saline,with 10 mice in each group.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LncRNA SNHG16,miR-302a-3p and E2F1.The cell viability was detected using the cell counting kit 8(CCK-8).The cell proliferation,invasion and apoptosis were detected using 5-ethynyl-2'-deoxyuridine(Edu),Transwell and in situ terminal transferase labeling technique(TUNEL).Results In cell experiment,the relative expression levels of LncRNASNHG16 in cell control group,cell experimental-H group,mimic-NC group,miR-302a-3p mimic group,oe-NC group and oe-LncSNHG16 group were 1.00±0.13,0.47±0.05,1.00±0.10,0.81±0.12,1.02±0.09 and 3.76±0.41,respectively;the relative expression levels of miR-302a-3p were 1.00±0.11,3.60±0.39,1.00±0.09,3.74±0.32,0.97±0.10 and 0.27±0.03,respectively;the relative expression levels of E2F1 mRNA were 1.00±0.14,0.23±0.02,1.00±0.10,0.33±0.03,0.95±0.08 and 2.18±0.25,respectively.Compared the cell experimental-H group with cell control group,compared miR-302a-3p mimic group with mimic-NC group and compared oe-LncSNHG16 group with oe-NC group,the above indicators were all statistically significantly different(P＜0.01,P＜0.001).The proliferation rates of cell control group,cell experimental-H group,WOG+oe-LncSNHG16 group and WOG+oe-LncSNHG16+miR-302a-3p mimic group were(95.04±10.14)％,(36.36±4.75)％,(97.64±11.04)％and(31.87±3.56)％,respectively;the number of invasive cells were(147.36±15.01),(43.31±6.25),(153.45±15.22)and(37.35±5.04),respectively;the apoptosis rates were(7.83±0.83)％,(35.77±4.38)％,(8.44±0.88)％and(29.35±3.09)％,respectively.There were statistically significant differences in the above indexes between the cell experimental-H group and the cell control group,between the WOG+oe-LncSNHG16 group and the cell experimental-H group,and between the WOG+oe-LncSNHG16 group and the WOG+oe-LncSNHG16+miR-302a-3p mimic group(all P＜0.001).In animal experiment,the tumor volume of animal control group and animal experimental-L,-M,-H groups were(2 554.50±280.41),(2 194.10±234.22),(1 512.40±166.75)and(1 146.20±124.32)mm3,respectively;the tumor mass was(2.13±0.28),(1.84±0.23),(1.65±0.21)and(1.32±0.14)g,respectively.There were statistically significant differences in the above indicators between experimental-L,-M,-H groups and animal control group(P＜0.05,P＜0.01,P＜0.001).Conclusion WOG can inhibit the proliferation and invasion of gastric cancer cells and promote their apoptosis.This may be related to the regulation of the LncRNA SNHG16/miR-302a-3p/E2F1 axis,which blocks the cell cycle.关键词
汉黄芩素/长链非编码核糖核酸小核仁核糖核酸宿主基因16/E2F转录因子1/胃癌/细胞周期/增殖/侵袭/凋亡Key words
woginostatin/long-chain non-coding ribonucleic acid small nucleolar ribonucleic acid host gene 16/E2F transcription factor 1/gastric cancer/cell cycle/proliferation/invasion/apoptosis分类
医药卫生引用本文复制引用
常毅刚,霍华英..汉黄芩素通过LncRNA SNHG16/miR-302a-3p/E2F1分子轴调控胃癌细胞生物学行为研究[J].中国临床药理学杂志,2026,42(7):954-961,8.基金项目
山西省中医药管理局科研课题计划基金资助项目(2024ZYYC040) （2024ZYYC040）
