中国临床药理学杂志2026，Vol.42Issue(7)：962-967,6.DOI:10.13699/j.cnki.1001-6821.2026.07.010
白花丹醌通过FNDC3B在舌鳞癌细胞恶性生物学行为与糖酵解中的作用研究
Research of the effects of plumbagin in the malignant biological behaviors and glycolysis of tongue squamous cell carcinoma cells through FNDC3B
摘要
Abstract
Objective To explore the mechanism of the inhibitory effects of plumbagin on the malignant biological behaviors and glycolysis of tongue squamous cell carcinoma cells through fibronectin type Ⅲ domain-containing protein 3B(FNDC3B).Methods Human tongue squamous cell carcinoma cells(TCA8113)in the logarithmic growth phase were obtained.TCA8113 cells were divided into blank group(normal cultured),experimental-L,M,and H group(TCA8113 cells were treated with 1,2 and 4 μmol·L-1 plumbagin),oe-NC group(TCA8113 cells were transfected with the negative control plasmid oe-NC),oe-FNDC3B group(TCA8113 cells were transfected with oe-FNDC3B),experimental-H+oe-NC group(based on experimental-H group,transfected with the oe-NC plasmid)and experimental-H+oe-FNDC3B group(based on experimental-H group,transfected with the oe-FNDC3B plasmid).Real-time quantitative polymerase chain reaction was used to detect FNDC3B mRNA relative expression levels.Cell viability was assessed using the methylthiazolyldiphenyl-tetrazolium bromide(MTT)assay.Cell invasion was evaluated using the Transwell assay.Immunofluorescence staining was performed to detect the protein relative expression levels of FNDC3B,hexokinase 2(HK2)and lactate dehydrogenase A(LDHA).Cell apoptosis was detected using the terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay.Results The numbers of invasive cells in blank group,experimental-H group,experimental-H+oe-NC group and experimental-H+oe-FNDC3B group were(89.71±13.59),(69.67±14.78),(62.33±10.11)and(158.23±20.95),respectively;the apoptosis rates were(10.07±2.41)％,(23.38±4.75)％,(20.65±5.43)％and(8.44±1.18)％,respectively;the relative expression levels of HK2 were 1.00±0.15,0.58±0.09,0.55±0.07 and 0.71±0.10,respectively;the relative expression levels of LDHA were 1.00±0.12,0.63±0.11,0.61±0.09 and 0.89±0.15,respectively;the relative expression levels of P53 were 1.00±0.15,1.59±0.31,1.64±0.28 and 1.25±0.22,respectively;the relative expression levels of P21 were 1.00±0.13,1.51±0.26,1.48±0.19 and 1.19±0.17,respectively;the relative expression levels of CDK6 were 1.00±0.13,0.28±0.04,0.33±0.06 and 0.79±0.12,respectively.There were statistically significant differences in the above indicators between experimental-H group and blank group,and between experimental-H+oe-FNDC3B group and experimental-H+oe-NC group(all P＜0.05).Conclusion Plumbagin may promote the apoptosis of tongue squamous cell carcinoma cells,and inhibit the invasion and glycolysis of cancer cells by regulating the P53/P21/CDK6 signaling pathway through FNDC3B.关键词
白花丹醌/舌鳞癌/含纤连蛋白Ⅲ型结构域蛋白3B/恶性行为/糖酵解Key words
plumbagin/tongue squamous cell carcinoma/fibronectin type Ⅲ domain-containing protein 3B/malignant behavior/glycolysis分类
医药卫生引用本文复制引用
闫倩,李琰,陈宏丽,张宇涛,张旭琴..白花丹醌通过FNDC3B在舌鳞癌细胞恶性生物学行为与糖酵解中的作用研究[J].中国临床药理学杂志,2026,42(7):962-967,6.基金项目
山西医科大学汾阳学院科研基金资助项目(2023L477) （2023L477）
