中国免疫学杂志2026，Vol.42Issue(5)：1059-1063,5.DOI:10.3969/j.issn.1000-484X.2026.05.006
山奈酚调控TLR4/NF-κB通路对类风湿关节炎大鼠炎症损伤的影响
Effect of kaempferol on inflammatory injury in rheumatoid arthritis rats by regulating TLR4/NF-κB pathway
摘要
Abstract
Objective:To investigate effect of kaempferol on inflammatory injury in rheumatoid arthritis(RA)rats by regulating TLR4/NF-κB pathway.Methods:RA rats were prepared by injecting a mixture of bovine type Ⅱ collagen acetic acid and equal amounts of Freund´s complete adjuvant or Freund´s incomplete adjuvant.Successfully prepared rats were randomly assigned into RA group,low and high doses kaempferol group,methotrexate group,and high kaempferol+activator group.Ten rats injected with same volume of physiological saline were used as control group.After intervention,degree of joint swelling in limbs of rats was evaluated,arthritis index score was performed,and ankle joint diameter was measured.ELISA was applied to detect levels of IL-6,IL-1β and TNF-α.Spleen and thymus were separated to calculate immune organ indexes.Synovial tissue of ankle joint was collected.HE staining was applied to detect pathological changes in synovial tissue,Western blot was applied to detect TLR4/NF-κB pathway related protein expression in synovial tissue.Results:Arthritis index,ankle joint diameter,serum IL-6,IL-1β,TNF-α levels,spleen index,thymus index,TLR4,p-NF-κB p65/NF-κB p65 expressions in synovial tissue of rats in RA group were obviously higher than control group(P<0.05).Arthritis index,ankle joint diameter,serum IL-6,IL-1β,TNF-α levels,spleen index,thymus index,TLR4,p-NF-κB p65/NF-κB p65 expressions in synovial tissue of rats in low and high doses kaempferol groups,and methotrexate group were obviously lower than RA group,and there were differences among different kaempferol groups(P<0.05).Arthritis index,ankle joint diameter,serum IL-6,IL-1β,TNF-α levels,spleen index,thymus index,TLR4,p-NF-κB p65/NF-κB p65 expressions in synovial tissue in high kaempferol+activator group were higher than high kaempferol group(P<0.05).Conclusion:Kaempferol alleviates inflammatory injury in RA rats by inhibiting TLR4/NF-κB pathway.关键词
类风湿关节炎/山奈酚/TLR4/NF-κB通路/炎症损伤Key words
Rheumatoid arthritis/Kaempferol/TLR4/NF-κB pathway/Inflammatory injury分类
医药卫生引用本文复制引用
牟义飞,汤正珍,李启飞,李颖,唐永恒..山奈酚调控TLR4/NF-κB通路对类风湿关节炎大鼠炎症损伤的影响[J].中国免疫学杂志,2026,42(5):1059-1063,5.基金项目
遵义市科技计划项目[遵市科合HZ字(2022)70号]. （2022）
