中国普通外科杂志2026，Vol.35Issue(3)：500-511,12.DOI:10.7659/j.issn.1005-6947.250286
PSMB2通过细胞外基质-受体相互作用通路影响胰腺导管腺癌预后的多维度分析
Multidimensional analysis of the prognostic impact of PSMB2 in pancreatic ductal adenocarcinoma via the ECM-receptor interaction pathway
摘要
Abstract
Background and Aims:Proteasome 20S subunit β2(PSMB2)has been implicated in tumor progression in multiple cancers;however,its expression pattern,prognostic value,and underlying mechanisms in pancreatic ductal adenocarcinoma(PDAC)remain unclear.This study aimed to investigate the clinical significance and potential mechanisms of PSMB2 in PDAC through integrated bioinformatics analysis and clinical validation. Methods:Expression profiles and clinical data from TCGA and GEO datasets(GSE62452 and GSE15471)were analyzed to evaluate PSMB2 expression and its prognostic significance.Kaplan-Meier survival analysis and Cox regression models were used.Immunohistochemistry was performed on 76 PDAC samples for validation.Gene Set Enrichment Analysis(GSEA)and correlation analyses were conducted to explore potential mechanisms.Immune cell infiltration was assessed using ssGSEA,and immunotherapy response was predicted using TCIA data.Drug sensitivity was analyzed based on the GDSC database. Results:IPSMB2 expression was significantly upregulated in PDAC tissues,and high expression was associated with poorer overall survival(all P<0.05).Multivariate Cox analysis identified PSMB2 as an independent prognostic factor.Immunohistochemical results were consistent with bioinformatics findings.PSMB2 expression correlated with lymph node metastasis,pathological stage,and differentiation.GSEA indicated enrichment in the ECM-receptor interaction pathway.Correlation analysis showed positive associations with multiple ECM-related genes.Additionally,PSMB2 was associated with immune cell infiltration and predicted immunotherapy response.Higher PSMB2 expression was also linked to increased sensitivity to several targeted agents. Conclusion:PSMB2 is overexpressed in PDAC and is associated with poor prognosis,serving as an independent prognostic biomarker.Its role may involve regulation of the ECM-receptor interaction pathway and tumor immune microenvironment,highlighting its potential as a therapeutic target.关键词
胰腺肿瘤/蛋白酶体20S亚基β2/预后/计算生物学/细胞外基质-受体相互作用通路Key words
Pancreatic Neoplasms/Proteasome 20S Subunit β2/Prognosis/Computational Biology/ECM-receptor Interaction Pathway分类
医药卫生引用本文复制引用
王大力,费浩然,孙丹,杨海深,胡伟..PSMB2通过细胞外基质-受体相互作用通路影响胰腺导管腺癌预后的多维度分析[J].中国普通外科杂志,2026,35(3):500-511,12.基金项目
江苏省连云港市卫生计生科技基金资助项目(201906,202102) （201906,202102）
江苏省连云港市科技局科技计划基金资助项目(SF2119) （SF2119）
江苏省连云港市第一人民医院科研基金资助项目(BS202003,LC04). （BS202003,LC04）
