中国人兽共患病学报2026，Vol.42Issue(3)：232-236,5.DOI:10.3969/j.issn.1002-2694.2026.00.057
大别班达病毒核蛋白单克隆抗体制备及其在抗原检测中的应用
Preparation of monoclonal antibodies to Dabie bandavirus nucleoprotein and applications in antigen detection
摘要
Abstract
This study was aimed at developing monoclonal antibodies(mAbs)to the nucleoprotein of Dabie bandavirus and identi-fying a specific mAb pair suitable for sandwich-based antigen detection with immunocolloidal gold,immunofluorescence chromatogra-phy,and enzyme-linked immunosorbent assay(ELISA).The recombinant nucleoprotein was expressed in HEK293 suspension cells,purified,and used to immunize BALB/c mice.Hybridoma technology was then used to generate mAbs.After immunization,mouse se-rum at a dilution of 1:10 000 showed strong antigen-antibody reactivity,with ELISA OD450 values ranging from 1.38 to 2.56.Through systematic screening,65 hybridoma cell lines were obtained,and one specific mAb pair with high specificity was successfully identi-fied.This antibody pair demonstrated positive binding to Dabie bandavirus antigen in immunocolloidal gold test strips,fluorescence immunochromatographic strips,and ELISA.The results confirmed the successful development of a practical mAb pair that can be ef-fectively applied for the detection of Dabie bandavirus antigen in multiple immunological assays.关键词
大别班达病毒/核蛋白/单克隆抗体/杂交瘤细胞/免疫胶体金/免疫层析/ELISAKey words
Dabie bandavirus/nucleoprotein/monoclonal antibody/hybridoma/immunogold/immunochromatography/ELISA分类
医药卫生引用本文复制引用
刘荣娇,何方玲,王娟,熊亚茹,湛志飞,李芳彩,蔡亮..大别班达病毒核蛋白单克隆抗体制备及其在抗原检测中的应用[J].中国人兽共患病学报,2026,42(3):232-236,5.基金项目
湖南省自然科学基金青年项目(No.2024JJ6265) （No.2024JJ6265）
湖南省卫健委高层次人才支持项目(No.20240304043) （No.20240304043）
湖南省卫健委一般课题(财政资助项目)(No.W20243067) （财政资助项目）
湖南省疾病预防控制中心青荷基金(重点)项目(No.QHJJ2023001) High-level Talent Support Project of Hunan Provincial Health Commission(No.20240304043) （重点）
The Youth Project of Hunan Natural Science Foundation(No.2024JJ6265) （No.2024JJ6265）
the Hunan Provincial Health Commission General Subject Funding Project(No.W20243067) （No.W20243067）
"Qinghe"Youth Cultivation Fund Project of Hunan Provincial Center for Disease Control and Prevention(No.QHJJ2023001). （No.QHJJ2023001）
