中国人兽共患病学报2026，Vol.42Issue(3)：257-262,6.DOI:10.3969/j.issn.1002-2694.2026.00.061
上海市浦东新区1例输入性基孔肯雅病毒鉴定及全基因组特征分析
Identification and whole-genome characterization of an imported Chikungunya virus case in Pudong District,Shanghai
摘要
Abstract
This study characterized the whole-genome sequence of an imported Chikungunya virus(CHIKV)in Shanghai,China,to clarify its molecular origin and assess local transmission risk.Serum RNA metatranscriptome sequencing was used for direct whole-genome capture from clinical samples and was complemented by phylogenetic analysis(MEGA 11.0,with 1 000bootstraps)and key amino acid variation screening(BioEdit V7.9.0).The results demonstrated successful acquisition of Shanghai's first imported CHIKV genome(11 891 bp;GenBank:PV805110.1)within 48 hours.Phylogenetic reconstruction confirmed an Asian genotype strain(CHIKV01/SH/CHN/2025)with 98.8％nucleotide identity to an Indonesian epidemic variant(Hu/Indonesia/NIID35/2015).Al-though the E1 protein carried the adaptive mutation K211E,critical sites for vector adaptation(E1-A226V,enhancing Aedes albopic-tus infection by 50-fold)and virulence(E1-D284E)retained wild-type configurations.This study established the first rapid whole-genome traceability of imported CHIKV in Shanghai,and confirmed its Indonesian origin and Asian genotype.The absence of key vector-adaptation mutations(E1-A226V)indicated low local transmission risk.关键词
基孔肯雅病毒/输入性病例/全基因组测序/系统进化分析/亚洲基因型Key words
Chikungunya virus/imported case/whole-genome sequencing/phylogenetic analysis/Asian genotype分类
医药卫生引用本文复制引用
张雪纯,应文欣,顾玙,陆馨晨,王筱,赵冰..上海市浦东新区1例输入性基孔肯雅病毒鉴定及全基因组特征分析[J].中国人兽共患病学报,2026,42(3):257-262,6.基金项目
上海市浦东新区科技发展基金事业单位民生科研专项(No.PKJ2025Y-23) Pudong District,Shanghai,Science and Technology Development Fund Public Service Research Grant(No.PKJ2025Y-23) （No.PKJ2025Y-23）
