中国人兽共患病学报2026，Vol.42Issue(3)：263-268,284,7.DOI:10.3969/j.issn.1002-2694.2026.00.062
基于CRISPR-Cas12b技术的登革病毒检测方法的建立
Establishment of a dengue virus detection method based on CRISPR-Cas12b
马银双 1刘静 1谢晓霞 2李娟娟 3杨阳 1张富强 1张蕾 1胡挺松1
作者信息
- 1. 中国人民解放军南部战区疾病预防控制中心,广州 510507
- 2. 广州医科大学公共卫生学院,广州 511436
- 3. 暨南大学基础医学与公共卫生学院,广州 510632
- 折叠
摘要
Abstract
This study was aimed at establishing a detection method for dengue virus(DENV)based on RPA-CRISPR/Cas12b tech-nology.We designed and synthesized RPA primers and sgRNAs targeting the conserved 3'-UTR region of the DENV genome,screened for optimal sgRNA and RPA primer combinations,and established the RPA-CRISPR/Cas12b detection system.Plasmid standards were constructed to evaluate sensitivity with varying plasmid concentrations.The established method was validated in 16 blood samples.The re-sults demonstrated a minimum detection limit of 1 copies/µL for DENV plasmid.The results for the 16 blood samples exhibited 100％con-cordance with RT-qPCR results.The established RPA-CRISPR/Cas12b-based DENV detection method is simple and rapid,and it exhib-its favorable specificity and sensitivity,thereby offering a novel strategy for faster,more portable identification of DENV.关键词
CRISPR-Cas12b/登革病毒/检测Key words
CRISPR-Cas12b/dengue virus/detection分类
医药卫生引用本文复制引用
马银双,刘静,谢晓霞,李娟娟,杨阳,张富强,张蕾,胡挺松..基于CRISPR-Cas12b技术的登革病毒检测方法的建立[J].中国人兽共患病学报,2026,42(3):263-268,284,7.
