中国人兽共患病学报2026，Vol.42Issue(4)：369-374,6.DOI:10.3969/j.issn.1002-2694.2026.00.065
2种卡介苗冻干疫苗的制备及其免疫原性的比较
Preparation of two types of freeze-dried Bacillus Calmette-Guérin vaccines and comparison of their immunogenicity
摘要
Abstract
Two types of BCG freeze-dried vaccines were prepared using different cultivation methods,and the immunogenicity of the two vaccines was compared.BCG strain was inoculated into Sutton medium and 7H9 complete medium respectively.BCG bacterial film vaccine(BCG-BV)and BCG bacterial liquid vaccine(BCG-BLV)were prepared by freeze-drying.BALB/c mice were immu-nized subcutaneously with the two vaccines,and the sera antibody levels were detected by ELISA.The lung T cell subsets of mice were detected by flow cytometry.The cytokines and inflammatory factors secreted by splenocytes stimulated in vitro were detected by ELISA.BCG-BLV contained a higher number of viable bacteria than BCG-BV.BCG-BLV induced an increase in the antibody level against Ag85B,and the subclass levels also showed a significant increase.BCG-BV induced a significant increase in the percentage of CD8+T cells in lung,whereas BCG-BLV decreased it.Both BCG-BV and BCG-BLV groups could induce the secretion of Th1(IFN-γ,IL-2)and Th2(IL-10)cytokines,and the levels of BCG-BLV immunized group were higher.Both BCG-BV and BCG-BLV could in-duce IL-1β,TNF-α and IL-6,which were characteristic cytokines of training immunity,and the BCG-BLV group induced a signifi-cantly higher level of IL-1β release.Different cultivation methods significantly influenced the immunogenicity of BCG.The freeze-dried BCG-BLV vaccine prepared in the enriched medium exhibited stronger immunogenicity.关键词
卡介苗/疫苗/免疫原性/训练免疫Key words
Bacillus Calmette-Guérin(BCG)/vaccine/immunogenicity/trained immunity分类
医药卫生引用本文复制引用
李嘉玲,常宇骁,康健,刘尚岐,王艺萌,宁唤唤,路延之,张琳娜,柏银兰..2种卡介苗冻干疫苗的制备及其免疫原性的比较[J].中国人兽共患病学报,2026,42(4):369-374,6.基金项目
国家自然科学基金(No.82272343,No.82502728) （No.82272343,No.82502728）
陕西省自然科学基金基础研究计划(No.2024JC-YBQN-0888) （No.2024JC-YBQN-0888）
陕西省重点研发计划一般项目(No.2024SF-YBXM-153) National Natural Science Foundation of China(No.82272343,No.82502728) （No.2024SF-YBXM-153）
Basic Research Program of Shaanxi Pro-vincial Natural Science Foundation(No.2024JC-YBQN-0888) （No.2024JC-YBQN-0888）
General Project of Shaanxi Key Research and Development Program(No.2024SF-YBXM-153) （No.2024SF-YBXM-153）
