中国人兽共患病学报2026，Vol.42Issue(4)：381-387,7.DOI:10.3969/j.issn.1002-2694.2026.00.023
福建省猪肉源金黄色葡萄球菌分离鉴定、耐药性及流行特征研究
Isolation,identification,resistance,and epidemiological characteristics of Staphylococcus aureus from pork sources in Fujian Province
摘要
Abstract
This study investigated the epidemiological characteristics,drug resistance,and drug resistance gene carriage of Staphylococcus aureus from pork in select areas of Fujian Province,to provide a reference for drug resistance risk assessment of Staphy-lococcus aureus from pork.Pork samples were collected from farmers´ markets,supermarkets,and slaughterhouses in select areas of Fujian Province from 2023 to 2024.Staphylococcus aureus was isolated with BP agar selective medium and identified by PCR.The sen-sitivity of the agar dilution method to 21 antimicrobial drugs was determined.Whole genome sequencing technology was used to detect drug resistance genes and perform multi-locus sequence typing(MLST).A total of 113 strains of Staphylococcus aureus were de-tected in 529 samples,and the total isolation rate was 21.4%.Antimicrobial susceptibility testing indicated that 64.6%of the bacte-ria were multidrug-resistant,and high resistance rates to tetracycline,sulfamethoxazole/trimethoprim,azithromycin,penicillin,and clindamycin(45.1%,45.1%,46.9%,50.4%and 57.5%,respectively)were observed.Genome sequencing of the isolates de-tected 35 drug resistance genes in nine categories,including macrolides,aminoglycosides,and tetracyclines.All isolates carried drug resistance genes,including special drug resistance genes such as mecA and cfr,notably,34.5%of the isolates carried 12 drug resistance genes.MLST typing analysis indicated 15 ST types,among which ST 7 was dominant(34.5%).Pork in the investigated areas of Fujian Province showed substantial contamination with Staphylococcus aureus that had high resistance rates to common clini-cal drugs and carried many drug resistance genes.Therefore,continued monitoring of Staphylococcus aureus from pork in this region should be strengthened.关键词
金黄色葡萄球菌/多重耐药/耐药基因/ST型Key words
Staphylococcus aureus/multidrug resistance/drug resistance genes/ST type分类
医药卫生引用本文复制引用
陈海贞,杨守深,叶欣颖,张家嘉,李兰,张妍,尹会方,郑新添,范克伟,黄翠琴..福建省猪肉源金黄色葡萄球菌分离鉴定、耐药性及流行特征研究[J].中国人兽共患病学报,2026,42(4):381-387,7.基金项目
福建省科技厅高校产学研合作项目(No.2024N5007) （No.2024N5007）
福建省第四批"创新之星"人才项目(No.[2023]110号) （No.[2023]110号）
福建省自然科学基金(No.2021J011099) （No.2021J011099）
动物源性人兽共患病防控福建省高校工程研究中心开放基金项目(No.2022K010) Fujian Provincial Department of Science and Technology University Industry-University-Research Cooperation Proj-ect(No.2024N5007) （No.2022K010）
The Fourth Batch of"Innovation Star"Talent Projects in Fujian Province(No.[2023]110) （No.[2023]110）
Natural Science Foundation Project of Fujian Province(No.2021J011099) （No.2021J011099）
Open Fund Project of Fujian Provincial University Engineering Research Center for the Prevention and Control of Zoonoses of Animal Origin(No.2022K010) （No.2022K010）
