中国人兽共患病学报2026，Vol.42Issue(4)：388-394,7.DOI:10.3969/j.issn.1002-2694.2026.00.033
2021-2024年北京市朝阳区临床分离肺炎克雷伯菌耐药表型及全基因组序列分析
Drug resistance phenotypes and whole genome sequences of clinical isolates of Klebsiella pneumoniae in Chaoyang District,Beijing,2021-2024
顾琳 1赵剑虹 1高艳 1黄平 1郭启明1
作者信息
- 1. 北京市朝阳区疾病预防控制中心,北京 100021
- 折叠
摘要
Abstract
This study was aimed at understanding the drug sensitivity of Klebsiella pneumoniae clinical isolates from hospitalized patients,and analyzing their drug resistance phenotypes,drug resistance genes,and high virulence genes.A total of 115 K.pneu-moniae strains clinically isolated from Chaoyang District,Beijing,from 2021 to 2024 were collected for drug sensitivity testing and whole genome sequencing.The original data were analyzed in the Microobench pathogen analysis workstation(without reference splic-ing).Statistical analysis was conducted in SPSS 27.0 software.The resistance rate of K.pneumoniae increased each year,and the resis-tance rates to 19 types of antibiotics exceeded 50%.The detection rate of carbapenem-resistant Klebsiella pneumoniae(CRKP)was 58.26%.Molecular typing revealed that the CC258 clone complex was the most prevalent sequence type in this region(45.22%),and all were ST11-type strains.A total of 14 strains of highly virulent K.pneumoniae(hvKP)were found,three of which were hyperviru-lent carbapenem-resistant K.pneumoniae(HCKP).The drug resistance of K.pneumoniae showed a continually increasing trend,and the emergence of HCKP suggested a need for vigilance regarding the evolutionary risks of"superbugs."Hospitals should strengthen clinical rational drug use,and integrate molecular monitoring and precise intervention to curb the spread of drug-resistant clones.关键词
肺炎克雷伯菌/耐药表型/全基因组测序/耐药基因/高毒力Key words
Klebsiella pneumoniae/phenotypic resistance/whole genome sequencing/antibiotic resistance gene/hypervirulence分类
医药卫生引用本文复制引用
顾琳,赵剑虹,高艳,黄平,郭启明..2021-2024年北京市朝阳区临床分离肺炎克雷伯菌耐药表型及全基因组序列分析[J].中国人兽共患病学报,2026,42(4):388-394,7.
