中国人兽共患病学报2026，Vol.42Issue(4)：395-402,8.DOI:10.3969/j.issn.1002-2694.2026.00.026
2022-2023年长沙市活禽市场H9N2亚型禽流感病毒遗传特征分析
Genetic characterization of H9N2 avian influenza virus from live poultry markets in Changsha,2022-2023
摘要
Abstract
This study analyzed the genetic characteristics of the hemagglutinin(HA)and neuraminidase(NA)genes of H9N2 subtype avian influenza virus(AIV)in live poultry markets(LPMs)in Changsha City during 2022-2023,to provide laboratory data to facilitate control of H9N2 subtype AIV outbreaks.A total of 809 environmental and poultry samples were collected from Changsha LPMs.Real-time fluorescence RT-PCR was used to detect influenza A virus and H9 subtype nucleic acids.Full-length gene amplifi-cation and sequencing were performed on H9-positive samples.Bioinformatics software was used for sequence alignment and genetic feature analysis.Nucleic acid detection revealed a 64.15%positivity rate for environmental AIV,and H9 showed the highest overall positivity rate(48.08%).Mixed infections exhibited the highest overall positivity rate of 5.07%for H5+H9.Genetic evolution analysis revealed that 29 isolates belonged to the B4.7.2 lineage,and five belonged to the B4.7.3 lineage,according to HA gene analysis.All NA genes belonged to the Y280-like subclade of the Eurasian lineage and showed close phylogenetic relationships with the candidate vaccine strain A/Anhui-Lujiang/39/2018.The HA protein cleavage site was PSRSSRGLF,in agreement with low pathogenic avian in-fluenza virus(LPAIV)characteristics.However,the presence of mutations Q226L(100%),I155T/N(17.65%/82.35%),and G192R(100%)suggested potential mammalian adaptation.The NA protein stem region exhibited a deletion at positions 63-65,but no muta-tions were observed at key drug resistance sites.Glycosylation site analysis revealed six conserved sites(29,141,298,305,313,and 492),whereas sites 105,145,206,and 218 were absent with respect to some reference strains,and site 313 was newly added.Antigenic site analysis revealed that 61.11%(11/18)of HA antigenic sites underwent mutations potentially leading to antigenic changes.Although H9N2 AIV from Changsha LPMs was classified as LPAIV,it exhibited mammalian adaptation mutations and anti-genic drift.Therefore,ongoing monitoring of its public health risks is necessary.关键词
活禽市场/禽流感病毒/H9N2亚型/遗传进化/分子特征Key words
live poultry market/avian influenza virus/H9N2 subtype/genetic evolution/molecular characterization分类
医药卫生引用本文复制引用
扶会媛,张如胜,黄政,罗璨,陈静芳,裴瑞青..2022-2023年长沙市活禽市场H9N2亚型禽流感病毒遗传特征分析[J].中国人兽共患病学报,2026,42(4):395-402,8.基金项目
湖南省卫生健康高层次人才重大科研专项(No.R2023169) （No.R2023169）
湖南省自然科学基金(No.2023JJ60399,No.2023JJ60070) Major Scientific Research Program for High-level Talents in Healthcare in Hunan Province(No.R2023169) （No.2023JJ60399,No.2023JJ60070）
Hunan Pro-vincial Natural Science Foundation(No.2023JJ60399,No.2023JJ60070) （No.2023JJ60399,No.2023JJ60070）
