中国人兽共患病学报2026，Vol.42Issue(4)：403-409,7.DOI:10.3969/j.issn.1002-2694.2026.00.030
郑州市健康人群携带耐碳青霉烯大肠埃希菌的特征分析
Characteristics of carbapenem resistant Escherichia coli carried by healthy individuals in Zhengzhou
摘要
Abstract
This study analyzed the positivity rates,antimicrobial resistance,and genomic characteristics of carbapenem resistant Escherichia coli(CREC)in the feces of healthy individuals in Zhengzhou city.Fecal samples were collected from healthy individuals.CREC strains were obtained through isolation,culture,and identification.The antimicrobial resistance profiles,resistance genes,virulence genes,plasmids,and multi-locus sequence typing(MLST)of the CREC strains were analyzed.A total of 12 CREC strains were isolated from 108 fecal samples,with a detection rate of 11.11%.The positivity rate was significantly higher in middle-aged indi-viduals(41-65 years,20.51%)than in young adults(18-40 years,5.80%)(χ2=5.46,P<0.05).The resistance rated to 16 antibiotics exceeded 80%.All strains were resistant to carbapenems,cephalosporins,ampicillin/sulbactam,and ampicillin/clavulanic acid.The resistance rates to minocycline(8.33%),fosfomycin w/G6P(16.67%),and norfloxacin(16.67%)were relatively low.All isolates were susceptible to tigecycline,nitrofurantoin,and amikacin.All strains were multidrug-resistant and carried one carbapenemase gene(11 carried blaNDM-5,and one carried blaNDM-1).The high-frequency co-occurring resistance genes were mdf(A)(100%),floR(83.33%),and qnrS1(75.00%).The main virulence genes carried were associated with pili,the iron transport system,and the type II/III secretion system.The plasmids IncX3 and IncF were detected in eight CREC strains.MLST analysis revealed that all 12 CREC strains belonged to different sequence types(STs).Therefore,healthy middle-aged individuals in Zhengzhou constitute a major CREC reservoir.All isolated CREC strains were highly resistant to most antibiotics and carried multiple resistance and virulence genes.CREC monitoring efforts in healthy people should be strengthened.关键词
耐碳青霉烯大肠埃希菌/耐药性/全基因组测序/耐药基因/毒力基因/耐药质粒/ST型Key words
carbapenem resistant Escherichia coli/drug resistance/whole genome sequencing/resistance genes/virulence genes/plasmids/sequence types(ST)分类
医药卫生引用本文复制引用
周鹏,王梦玉,朱刘丹,赵雪蕾,张晓甍,刘子瑜,程春荣..郑州市健康人群携带耐碳青霉烯大肠埃希菌的特征分析[J].中国人兽共患病学报,2026,42(4):403-409,7.基金项目
河南省医学科技攻关计划项目(No.LHGJ20220901). Henan Provincial Medical Science and Technology Research Plan(No.LHGJ20220901). （No.LHGJ20220901）
