中国水产科学2026，Vol.33Issue(4)：88-100,13.DOI:10.12264/JFSC2025-0309
传染性脾肾坏死病毒感染促进鳜脑组织细胞脂肪酸合成及β-氧化
Infectious spleen and kidney necrosis virus promotes fatty acid synthesis and β-oxidation in brain cells of Siniperca chuatsi
摘要
Abstract
We used transcriptomics,metabolomics,inhibitor experiments,reverse transcription quantitative PCR(RT-qPCR),quantitative PCR(qPCR),and western blotting(WB)to investigate the regulatory effects of infectious spleen and kidney necrosis virus(ISKNV)infection on fatty acid metabolism in Chinese perch brain(CPB)cells by exploring the relationship between ISKNV replication and host cell lipid metabolism.Multi-omics integrative analysis revealed a significant increase in the transcriptional expression of fatty acid synthesis-related genes(ACC1,FASN)and fatty acid oxidation-related genes(ATGL,HSL,and CPT)at 24 and 72 hours post-infection(hpi)with ISKNV.The content of various fatty acid metabolites increased at 24 hpi but decreased significantly at 72 hpi.Further experiments confirmed that at 36 hpi of ISKNV infection,the mRNA and protein levels of key fatty acid synthesis proteins(ACLY,ACC1,and FASN)were significantly upregulated;the mRNA and protein levels of key fatty acid oxidation proteins(ATGL and CPT1)and rate of fatty acid oxidation were significantly increased at 72 hpi.Inhibition of key fatty acid synthesis enzymes ACC1 and FASN using TOFA and C75 significantly suppressed the transcription and protein expression of ISKNV MCP and copy number in the extracellular supernatant at 72 hpi.Inhibition of key fatty acid oxidation enzyme CPT1 with Etomoxir also significantly reduced MCP protein expression and extracellular supernatant copy number at 72 hpi.These results indicate that ISKNV infection activates fatty acid synthesis and oxidation in CPB cells to support its own replication,providing a theoretical basis for an in-depth understanding of the pathogenic mechanism of ISKNV and antiviral strategies.关键词
鳜/传染性脾肾坏死病毒/脂肪酸合成/脂肪酸β-氧化/病毒复制Key words
Siniperca chuatsi/infectious spleen and kidney necrosis virus/fatty acid synthesis/fatty acid β-oxidation/virus replication分类
农业科技引用本文复制引用
朱怡霖,李宁求,张晓婷,牛银杰,马宝福,罗霞,林强,梁红茹,付小哲..传染性脾肾坏死病毒感染促进鳜脑组织细胞脂肪酸合成及β-氧化[J].中国水产科学,2026,33(4):88-100,13.基金项目
国家自然科学基金项目(32273181) （32273181）
广东省现代农业产业技术体系创新团队建设项目(2024CXTD26) （2024CXTD26）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2023TD48). （2023TD48）
