中国水产科学2026，Vol.33Issue(4)：101-112,12.DOI:10.12264/JFSC2025-0249
基于镜检与DNA宏条形码技术的恰甫其海伊犁鲈食性特征研究
Study on dietary characteristics of Perca schrenkii in Qiapuqihai Reservoir based on microscopy and DNA metabarcoding
摘要
Abstract
As an economically dominant fish species in tributaries and reservoirs of the Ili River Basin in Xinjiang,studying the feeding habits of Perca schrenkii is essential to clarify its prey and trophic level.We used a combination of microscopic examination and DNA metabarcoding to systematically analyze the feeding intensity,dietary composition,and seasonal variations of Perca schrenkii in Qiapuqihai Reservoir,Xinjiang.The results indicated that the annual feeding rate of Perca schrenkii reached 93%,with the highest feeding intensity observed in autumn,followed by spring;the lowest was observed in summer.Microscopic examination revealed that the diet primarily consisted of zooplankton(Daphnia and Sinocalanus)and benthic organisms(Chironomus),followed by fish(Abbottina rivularis and Carassius auratus gibelio).Microscopy complemented by DNA metabarcoding identified prey such as small fish and revealed eight additional genera of microorganisms,resulting in a 51.5%genus-level overlap and demonstrating significant methodological complementarity.Similarity analysis of prey organisms across seasons revealed significant differences in dietary composition(P<0.05).Non-metric multidimensional scaling results indicated distinguishable distributions of samples among seasons,with a richer prey diversity in spring and a relatively simpler composition in summer.The results of this study demonstrate that combining both methods significantly enhances prey identification comprehensiveness,thereby systematically revealing the ecological feeding characteristics of Perca schrenkii.These findings provide a theoretical foundation for understanding energy flow in the Qiapuqihai Reservoir aquatic ecosystem and managing fishery resources.关键词
伊犁鲈/镜检分析法/DNA宏条形码技术/食性特征/恰甫其海Key words
Perca schrenkii/microscopic examination/DNA metabarcoding/feeding habits/Qiapuqihai Reservoir分类
农业科技引用本文复制引用
姚春江,凡迎春,潘子涵,徐东坡,罗治远..基于镜检与DNA宏条形码技术的恰甫其海伊犁鲈食性特征研究[J].中国水产科学,2026,33(4):101-112,12.基金项目
国家重点研发计划项目(2023YFD2400900) （2023YFD2400900）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2023TD65). （2023TD65）
