中国水稻科学2026，Vol.40Issue(3)：351-359,9.DOI:10.16819/j.1001-7216.2026.250604
矮格拉/徐稻10号DH群体的SNP标记图谱构建及耐盐QTL定位分析
Construction of a SNP-Based Genetic Map and QTL Mapping for Salt Tolerance in a Rice DH Population Derived from Aigela and Xudao 10
摘要
Abstract
[Objective]Soil salinization is an important abiotic factor affecting agricultural production in China,and the identification of salt tolerance-related genes/QTLs in rice is crucial for ensuring stable rice production in saline-alkali soils.[Method]Aigela and Xudao 10,which exhibit significant differences in salt tolerance related traits,were used as parent plants to create a doubled haploid(DH)population.Salt tolerance was identified under field and hydroponic conditions.Genotyping of the targeted population was performed using the rice 1 K mGPS SNP chip.Phenotypic data combined with genotype data of DH population were used for QTL mapping with the QTL IciMapping 4.2 software.[Result]Through QTL mapping analysis,a total of 22 salt tolerance related QTLs were detected,including 10 QTLs for grain yield per plant,4 for grains per panicle,2 for root length,and 6 for root dry weight.These QTLs are located on chromosomes 1,6,8,10,11,and 12,explaining phenotypic variation of 3.37%-15.52%with LOD values ranging from 4.04 to 9.65.Based on the gene annotation,Arabidopsis homologous gene comparison,spatiotemporal expression analysis and sequence comparison within the mapping interval of qST11,a candidate gene(LOC_Os11g30560)encoding a hydroxysteroid dehydrogenase(OsHSD1)was identified.[Conclusion]Among the 22 QTLs detected in this study,11 were known genes or loci related to salt tolerance,and the remaining 11 were newly identified.Meanwhile,the candidate gene analysis was conducted on the newly identified salt tolerance QTL(qST11),laying a foundation for cloning of salt-tolerance related genes and deciphering their genetic regulatory networks.关键词
耐盐相关性状/双单倍体群体/QTL定位/候选基因Key words
salt tolerance-related trait/doubled haploid population/QTL mapping/candidate gene引用本文复制引用
刘艳,周群,王德荣,陈庭木,刘金波,卢百关,徐大勇,王宝祥,李景芳,迟铭,章雨沁,孙志广,李健,杨波,徐波,邢运高..矮格拉/徐稻10号DH群体的SNP标记图谱构建及耐盐QTL定位分析[J].中国水稻科学,2026,40(3):351-359,9.基金项目
国家现代农业产业技术体系项目(CARS-01-97) （CARS-01-97）
生物育种钟山实验室项目(ZSBBL-KY2024-01) （ZSBBL-KY2024-01）
江苏省自然科学基金面上项目(BK20221276) （BK20221276）
江苏省种业振兴揭榜挂帅项目(JBGS[2021]043) （JBGS[2021]043）
连云港市财政专项(QNJJ2411). （QNJJ2411）
