中国饲料Issue(9)：22-29,8.DOI:10.15906/j.cnki.cn11-2975/s.2024120010-04
茅苍术地上部分总黄酮纯化工艺及抑菌活性研究
Purification process and antibacterial activity of total flavonoids from the aerial part of Atractylodes lancea
摘要
Abstract
This experiment used the aerial part of Atractylodes lancea as the material,total flavonoids were extracted by ultrasonic method.Then the optimal resin model was selected based on the results of static adsorption and static desorption experiments;Using adsorption rate and desorption rate as indicators,the effects of sample concentration,sample loading speed,sample loading volume,and eluent concentration,elution speed,and eluent volume on the purification process of total flavonoids were investigated,and the total flavonoid purification process was optimized through orthogonal design.In addition,the antibacterial activities of total flavonoids against Staphylococcus aureus and Escherichia coli were studied using the agar plate method.The results showed that D101 macro-porous resin was the most suitable one.The purification process involved a sample concentration of 1.20 mg/mL,a sample loading rate of 1 BV/h,a sample volume of 30 mL,and 60 mL of 80%ethanol as the eluent.The elution rate was controlled at 1 BV/h.Using this purification condition,the total flavonoid purity could reach 48.2%.The total flavonoids obtained had a significant antibacterial effect on Staphylococcus aureus,with a minimum inhibitory concentration of 2.26 mg/mL.关键词
茅苍术/地上部分/总黄酮/大孔树脂/正交法/抑菌Key words
Atractylodes lancea/the aboveground parts/total flavonoid/macro-porous resins/orthogonal design/antibacterial activity分类
农业科技引用本文复制引用
张阿琴,朱静,谷巍,黄美子,王升..茅苍术地上部分总黄酮纯化工艺及抑菌活性研究[J].中国饲料,2026,(9):22-29,8.基金项目
江苏农牧科技职业学院校级科研项目"茅苍术地上部分化学成分及抗菌、抗炎活性研究"(NSF0222CB09) （NSF0222CB09）
江苏农牧科技职业学院校级科研团队项目(NSF2024TC02) （NSF2024TC02）
江苏(泰州)现代农业(特粮特经)科技综合示范基地项目(JATS[2022]301) （泰州）
国家中医药管理局"中药资源保障能力提升项目(2023)名贵中药资源可持续利用能力建设项目"(2060302) （2023）
