中国实验动物学报2026，Vol.34Issue(4)：496-504,9.DOI:10.3969/j.issn.1005-4847.2026.04.003
一种改良的小鼠铜绿假单胞菌慢性肺部感染模型的建立与评价
Establishment and evaluation of an improved mouse model of chronic pulmonary Pseudomonas aeruginosa infection
摘要
Abstract
Objective To establish and systematically evaluate a mouse chronic pulmonary Pseudomonas aeruginosa(PA)infection model,providing a reliable method for establishing a model for drug treatment of chronic PA infections.Methods A feasible modified method was used to prepare PA-coated alginate microbeads.A chronic PA infection model was established in C57BL/6J mice using tracheal intubation instillation.Model success was systematically evaluated by observing changes in physiological state,blood routine analysis,bacterial load measurements in bronchoalveolar lavage fluid(BALF)and lung tissue,measurement of inflammatory cytokine expression,and histopathological section analysis.Results After intratracheal instillation of alginate microbeads coated with PA,the infected mice showed poorer physiological condition and higher mass loss compared with mice in the normal and the saline-alginate microbead groups.PA was cultured from model mouse lung tissue homogenate and BALF,and the number of white cells and neutrophils in the blood significantly increased(P＜0.05).Additionally,levels of the pro-inflammatory cytokines tumor necrosis factor-α(TNF-α)and procalcitonin(PCA)in BALF were significantly elevated.Hematoxylin-eosin(HE)and Masson staining of lung tissue pathological sections revealed significant inflammatory cell infiltration and pathological changes in the model group.Conclusions PA-coated alginate microbeads were successfully prepared using a modified method,and a chronic pulmonary PA infection model was established in mice through tracheal intubation instillation,providing a practical and feasible experimental animal model for PA infection drug treatment research.关键词
铜绿假单胞菌/海藻酸盐珠/慢性肺部感染/气管插管/动物模型Key words
Pseudomonas aeruginosa/alginate beads/chronic pulmonary infection/tracheal intubation/animal model分类
生物科学引用本文复制引用
王前梅,姚璞,王瑜,孙凤军..一种改良的小鼠铜绿假单胞菌慢性肺部感染模型的建立与评价[J].中国实验动物学报,2026,34(4):496-504,9.基金项目
国家自然科学基金青年项目(82102448).Funded by Young Scientists Fund of the National Natural Science Foundation of China(82102448). （82102448）
