中国实验动物学报2026，Vol.34Issue(4)：505-518,14.DOI:10.3969/j.issn.1005-4847.2026.04.004
人流刮宫与单纯机械损伤构建子宫内膜损伤大鼠模型的评价与比较
Evaluation and comparison of rat models of endometrial injury induced by artificial abortion and simple mechanical injury
摘要
Abstract
Objective Compared with the simple mechanical injury method and the artificial abortion mechanical injury method,to explore the animal model which is more in line with the clinical etiology and disease characteristics of endometrial injury and to explore the reasons why intrauterine operation during pregnancy is more likely to cause injury.Methods 42 female rats were randomly divided into 3 groups:sham group(n=6),simple mechanical injury(S)group(n=18),and artificial abortion injury(A)group(n=18),except for the Sham group,the other two groups were divided into 3 subgroups,which were taken in the first,second and third estrous period after operation.The histopathology changes of endometrium were observed by HE staining,the degree of endometrial fibrosis was observed by Masson staining,and the expression of CD31 and LIF in rat uterine tissue was detected by immunohistochemistry The expression of CD68 in endometrium was detected by immunofluorescence.Serum levels of inflammatory factors and hormones were measured by ELISA,and the expression of CD68,CD86 and TGF-β in endometrium was detected by Western Blot.Results(1)In the third estrous phase after surgery,the pathological changes of the uterus in group S were small,and the changes of endometrial thickness,epithelial cells and macrophages were not significant,there was no significant difference in the level of inflammation between the group S and Sham group.Only the level of fibrosis and the ability of vascular regeneration were consistent with the clinical characteristics of endometrial injury.However,in the group A,the endometrium became thinner,the number of glands decreased,the luminal epithelial cells decreased significantly,the expression of macrophages increased and was accompanied by obvious inflammatory response,and the level of uterine fibrosis increased significantly,the ability of vascular regeneration was decreased.Four dimensions were in line with the clinical characteristics.(2)After modeling with both methods,the level of endometrial fibrosis inereased with the increase of injury time and the level of serum inflammatory factors decreased with the inerease of injury time,however,the level of inflammatory factors decreased more slowly and the inflammatory reaction lasted longer in group A.Conclusions The artificial abortion injury model is more in line with the current understanding of the clinical etiology and disease characteristics of human endometrial injury,the reason why the uterine cavity operation during pregnancy is more vulnerable to injury may be related to the sustained high expression of macrophages,which leads to the chronic inflammation of the endometrium.关键词
子宫内膜损伤/动物模型/子宫巨噬细胞/纤维化/慢性炎症Key words
endometrial injury/animal model/uterine macrophages/fibrosis/chronic inflammation分类
生物科学引用本文复制引用
何斯怡,童若一,周海忆,李玖利,关永格,宋阳..人流刮宫与单纯机械损伤构建子宫内膜损伤大鼠模型的评价与比较[J].中国实验动物学报,2026,34(4):505-518,14.基金项目
广州中医药大学校院联合科技创新基金(GZYZS2024U13),广东省自然科学基金面上项目(2024A1515012194).Funded by University-Hospital Joint Fund Project of Guangzhou University of Chinese Medicine(GZYZS2024U13),General Program of Guangdong Basic and Applied Basic Research Foundation(2024A1515012194). （GZYZS2024U13）
