中国实验动物学报2026，Vol.34Issue(4)：519-530,12.DOI:10.3969/j.issn.1005-4847.2026.04.005
肾虚血瘀型Kümmell病大鼠模型的构建与验证
Establishment and characterization of a rat model simulating Kümmell disease with kidney deficiency and blood stasis syndromes
摘要
Abstract
Objective To establish an animal model of Kümmell disease(KD)characterized by kidney deficiency and blood stasis,providing a foundation for exploring therapeutic targets and underlying pathophysiological mechanisms.Methods Forty SD rats were randomly assigned to normal,osteoporosis(OP),sham operation(Sham),control,and model(KD)groups.Rats in the KD group underwent ovariectomy to induce kidney deficiency,followed 2 months later by creation of coccygeal bone defects and application of mechanical stress for 4 weeks.One week after bone defect surgery,subcutaneous injections of adrenaline hydrochloride were administered to induce blood stasis.Control group rats underwent ovariectomy and bone defect surgery but received 0.9％sodium chloride solution.During the experiment,body temperature,body mass,food and water intake,and tongue characteristics were monitored.After model establishment,behavioral assessments,imaging analyses,hemorheological testing,and histopathological examinations were performed.Results Compared with the sham and control groups,KD group rats showed elevated body temperature,mass loss,reduced food intake,purple tongues with tortuous sublingual veins,dry fur,lethargy,and impaired mobility.Imaging revealed cortical thinning,sparse and fractured trabeculae,and vacuum cleft signs in vertebral bodies.Hemorheological evaluation showed significantly increased whole blood viscosity at low,medium,and high shear rates,increased plasma viscosity,elevated fibrinogen levels,and shortened thrombin time,prothrombin time,and activated partial thromboplastin time.Serum calcium and phosphorus levels decreased,while bone turnover markers increased.Thyroid hormone and estradiol levels were significantly lower than in the sham group.Histopathological examination showed KD group disrupted trabecular structure and reduced new bone formation compared with the control group.Conclusions Combined ovariectomy,adrenaline-induced blood stasis,and mechanical stress successfully establish a rat model of KD with kidney deficiency and blood stasis syndrome,providing a useful platform for mechanistic studies and therapeutic evaluation.关键词
Kümmell病/肾虚血瘀/动物模型/病证结合/卵巢摘除/骨质疏松症Key words
Kümmell disease/kidney deficiency and blood stasis/animal model/combination of disease and syndrome/ovariectomy/osteoporosis分类
生物科学引用本文复制引用
王东平,张顺聪,刘畅,朱一豪,古淑灵,成品莹,高逸涵,冯紫淇,李子彬,李永贤..肾虚血瘀型Kümmell病大鼠模型的构建与验证[J].中国实验动物学报,2026,34(4):519-530,12.基金项目
广东省中医药管理局(20241091),中华中医药学会青年人才提升项目(2022-QNRC2-B11),广东省中医药管理局科研项目(20251106).Funded by the Administration of Traditional Chinese Medicine of Guangdong Province,China(20241091),Chinese Society of Traditional Chinese Medicine Youth Talent Lifting Project(2022-QNRC2-B11),Guangdong Provincial Administration of Traditional Chinese Medicine Research Project(20251106). （20241091）
