中国实验方剂学杂志2026，Vol.32Issue(10)：103-111,9.DOI:10.13422/j.cnki.syfjx.20252308
茵芪三黄解毒汤抑制中性粒细胞-胞外诱捕网的抗肝纤维化作用机制
Anti-hepatic Fibrosis Mechanism of Yinqi Sanhuang Jiedu Decoction via Inhibiting Neutrophils and Neutrophil Extracellular Traps
摘要
Abstract
Objective:To verify the therapeutic effect of Yinqi Sanhuang Jiedu decoction(YQSH)on carbon tetrachloride(CCl4)-induced hepatic fibrosis in mice,and to explore whether its effect was related to the inhibition of neutrophil infiltration and the formation of neutrophil extracellular traps(NETs).Methods:The 36 C57BL/6J mice were randomly divided into control group,model group,positive drug silybin(SF)group(55 mg·kg-1·d-1),YQSH-L group,YQSH-M group,and YQSH-H group(8.325,16.65,33.3 g·kg-1·d-1,respectively),n=6 in each group.Except for the control group,mice in all other groups were intraperitoneally injected with CCl4 to induce hepatic fibrosis.After successful modeling,each drug administration group was given the corresponding drugs by gavage for eight weeks.Hematoxylin-eosin(HE)staining,Sirius red staining and Masson staining were used to observe the pathological changes of liver tissue.Liver elasticity was detected by a color Doppler ultrasound system.Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction(Real-time PCR)were performed to detect the protein expression and mRNA levels of C-X-C motif chemokine ligand 1(CXCL1),CXCL2 and CXCL5.Neutrophil levels were detected by flow cytometry.The expression of neutrophil elastase(NE)and myeloperoxidase(MPO)positive protein was observed by immunofluorescence.The contents of MPO,NE and CitH3 were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the control group,the liver of the model group showed obvious inflammatory cell infiltration and collagen deposition,and the liver elasticity,CXCL1,CXCL2,CXCL5 expression,neutrophil level,and MPO,NE and CitH3 levels were significantly increased(P＜0.05,P＜0.01).Compared with the model group,inflammatory cell infiltration and collagen deposition in the liver tissue of mice were reduced after YQSH treatment.Moreover,the liver elasticity was reduced(P＜0.01).The protein expression(P＜0.01)and mRNA level of CXCL1,CXCL2 and CXCL5 were decreased(P＜0.05,P＜0.01).The neutrophil level was decreased(P＜0.01),the expression of MPO and NE positive protein was significantly decreased(P＜0.05,P＜0.01),and the levels of MPO,NE and CitH3 were decreased(P＜0.05,P＜0.01).Conclusion:The anti-hepatic fibrosis effect of YQSH may be related to its inhibition of chemokines(CXCL1,CXCL2,CXCL5),reduction of neutrophil infiltration,and inhibition of NETs generation.关键词
肝纤维化/茵芪三黄解毒汤/中性粒细胞/中性粒细胞胞外诱捕网/趋化因子Key words
hepatic fibrosis/Yinqi Sanhuang Jiedu decoction/neutrophil/neutrophil extracellular trap net/chemokines分类
医药卫生引用本文复制引用
李彦波,雷超,武庆娟,吕文良..茵芪三黄解毒汤抑制中性粒细胞-胞外诱捕网的抗肝纤维化作用机制[J].中国实验方剂学杂志,2026,32(10):103-111,9.基金项目
中央高水平中医医院临床研究和成果转化能力提升项目-中医药临床循证研究专项(HLCMHPP2023086) （HLCMHPP2023086）
国家自然科学基金面上项目(82374332) （82374332）
