中国兽医杂志2026，Vol.62Issue(5)：19-28,10.DOI:10.20157/j.cnki.zgsyzz.2026.05.003
复合益生菌对艾美耳球虫感染断奶羔羊生产性能、免疫功能和肠道菌群的影响
Effects of Compound Probiotics on Growth Performance,Immune Function,and Intestinal Microbiota of Weaned Lambs Infected with Eimeria
摘要
Abstract
This study aimed to investigate the effects of compound probiotics on growth performance,immune function,intestinal microbiota,and fecal oocyst shedding in weaned lambs experimentally infected with Eimeria.Forty 50-day-old weaned Hu lambs with similar body weight and health status were randomly assigned to four groups:Control(CON)group,Eimeria-infected(CI)group,Compound probiotics(CP)group,and Diclazuril-treated(DT)group.Initial body weight,final body weight,and feed intake were recorded to calculate average daily gain(ADG),average daily feed intake(ADFI),and feed conversion ratio(FCR).At the end of the 35-day trial,blood samples were collected to measure serum biochemical parameters and immune-related indices.Immune organs,including the liver and thymus,were weighed to calculate organ indices.During the experiment,fecal scores were recorded periodically to assess diarrhea,and oocysts per gram of feces(OPG)were counted.Rectal contents were collected,and microbial community structure was analyzed using Illumina MiSeq high-throughput sequencing.The results showed that the coccidial infection model was successfully established.Compared with the CI group,lambs in the CP group showed significantly increased ADG and ADFI(P<0.01).Serum total protein(TP),albumin(ALB),interleukin-2(IL-2),and the numbers of CD4⁺and CD8+T lymphocytes were significantly increased(P<0.05 or P<0.01),while creatinine(CREA)levels were significantly decreased(P<0.05).In addition,dietary supplementation with compound probiotics reversed the increases in liver weight and liver index as well as the decrease in thymus weight caused by Eimeria infection(P<0.05),significantly alleviated diarrhea(P<0.05),and reduced oocyst shedding.Regarding intestinal microbiota,the Chao1 and Shannon indices were significantly increased(P<0.01),indicating improved microbial richness and diversity,and the microbial community structure was optimized.These results suggest that compound probiotics can alleviate growth inhibition and diarrhea caused by coccidial infection in lambs by enhancing immune function,increasing microbial abundance,and optimizing gut microbiota structure.Their effects are comparable to those of diclazuril,while avoiding potential side effects associated with chemical drugs.关键词
复合益生菌/断奶羔羊/生产性能/免疫功能/肠道菌群/艾美耳球虫/感染Key words
compound probiotics/weaned lambs/growth performance/immune function/intestinal microbiota/Eimeria/infection分类
农业科技引用本文复制引用
赵梦瑶,徐曾浩,黎露露,孔亮,张红英,毛赛,张素梅,朱雷,杨明凡..复合益生菌对艾美耳球虫感染断奶羔羊生产性能、免疫功能和肠道菌群的影响[J].中国兽医杂志,2026,62(5):19-28,10.基金项目
河南省重点研发专项(231111111600) （231111111600）
