中国兽医杂志2026，Vol.62Issue(5)：65-71,7.DOI:10.20157/j.cnki.zgsyzz.2026.05.009
盐酸氯苯胍遗传毒性评价研究
Genotoxicity Assessment of Robenidine Hydrochloride
摘要
Abstract
Robenidine hydrochloride(ROBH)is a chemically synthesized anticoccidial drug widely used in animal husbandry because of its good efficacy and relatively low production cost.At present,information on the potential genotoxicity of ROBH is limited and remains highly controversial.To clarify the genotoxicity of ROBH,the Ames test,mouse bone marrow cell micronucleus test,and single-cell gel electrophoresis assay were employed to investigate its genotoxic effects at the gene,chromosome,and DNA levels,respectively.The results showed that,compared with the sterile distilled water group,the average numbers of mutant colonies in the low-dose ROBH groups(200,40,and 8 μg/plate)of the four test strains were not significantly different(P>0.05),whereas the average numbers of mutant colonies in the high-dose ROBH groups(5 000 and 1 000 μg/plate)were significantly decreased(P<0.05).Compared with the Negative control group,the micronucleus rates of polychromatic erythrocytes(PCEs)in the 195.00 and 97.50 mg/(kg·bw)ROBH dose groups were significantly increased(P<0.01).Obvious comet tailing was observed in hepatocellular carcinoma cell line 2(HepG2)cells treated with 20 and 25 μmol/L ROBH.Compared with the Control group,the percentages of tail DNA in HepG2 cells in the 20 and 25 μmol/L ROBH groups were significantly increased(P<0.01).These results indicate that low-dose ROBH is non-mutagenic to the test strains,whereas high-dose ROBH shows bacterial toxicity to the test strains.ROBH can induce chromosomal damage and DNA strand breaks in mammalian cells,demonstrating clear genotoxicity.The findings of this study provide important evidence for the re-evaluation of the safety of ROBH.关键词
盐酸氯苯胍(ROBH)/Ames试验/小鼠骨髓细胞微核试验/单细胞凝胶电泳试验/遗传毒性Key words
robenidine hydrochloride(ROBH)/Ames test/mouse bone marrow cell micronucleus assay/single-cell gel elec-trophoresis assay/genotoxicity分类
农业科技引用本文复制引用
梁云涛,张瑞琛,曾子康,叶姝怡,李红丽,汤树生..盐酸氯苯胍遗传毒性评价研究[J].中国兽医杂志,2026,62(5):65-71,7.基金项目
国家自然科学基金(32473083) （32473083）
