中国瓜菜2026，Vol.39Issue(5)：7-22,16.DOI:10.16861/j.cnki.zggc.2025.0758
基于GWAS分析挖掘甜瓜熟性相关候选基因
Genome-wide association study identifies candidate genes associated with melon ripening traits
摘要
Abstract
To identify the genetic loci and candidate genes associated with melon maturity traits and thereby accelerate the breeding of novel early-maturing melon cultivar,in this study,151 muskmelon accessions displaying diverse maturity characteristics were analyzed using a whole-genome re-sequencing approach to perform a genome-wide association stud-ies(GWAS)on maturity-related traits.The primary objective was to detect significantly associated loci,thereby establish-ing a theoretical basis for the development of early-maturing melon cultivar.The results indicate that among the 151 ana-lyzed accessions,36 exhibited early maturity characteristics.A total of 2 165 342 high-quality SNPs loci were identified,and the MLM(QK)model identified 55 SNP loci significantly associated with melon ripening traits.Within the 100 kb flanking regions surrounding these loci,a total of 240 alleles were identified.Integrated functional annotation and enrich-ment analyses revealed that the candidate genes are predominantly involved in phenylpropanoid biosynthesis,secondary metabolite biosynthesis,fatty acid metabolism,amino acid biosynthesis,and plant hormone signal transduction pathways.The two most significant SNP loci are located on chromosomes 4 and 12,respectively.The genes closely associated with the SNP locus on chromosome 4 are MELO3C009181 and MELO3C009179,whereas the gene linked to the SNP locus on chromosome 12 is MELO3C002391.Notably,MELO3C009181(LAX2)encodes an auxin transporter-like protein,and MELO3C002391(PER7)encodes a peroxidase,both of which represent potential candidate genes involved in the regula-tion of melon ripening.This study establishes a molecular foundation for ripening-related breeding and the development of early-maturing melon cultivar.关键词
甜瓜/熟性/早熟/全基因组关联分析Key words
Melon/Maturity/Early maturation/Genome-wide association study分类
农业科技引用本文复制引用
王红梅,黄金艳,解华云,李桂芬,覃斯华,张德梅,洪日新,何毅,柳唐镜,叶云峰..基于GWAS分析挖掘甜瓜熟性相关候选基因[J].中国瓜菜,2026,39(5):7-22,16.基金项目
广西重点研发计划(桂科AB21196045,桂科农AB2506910004) （桂科AB21196045,桂科农AB2506910004）
广西自然科学基金(2023GXNSFAA026437) （2023GXNSFAA026437）
国家自然科学基金(32260677) （32260677）
国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-2024-17-02) （nycytxgxcxtd-2024-17-02）
国家现代农业产业技术体系建设专项(CARS-25) （CARS-25）
广西农业和乡村振兴人才培养支持专项农业科技青年人才托举工程项目(GXQNTJ242104) （GXQNTJ242104）
广西农业科学院专项(桂农科2024ZX26,桂农科2026YT079) （桂农科2024ZX26,桂农科2026YT079）
广西农业科学院博士后基金(桂农科博2024058) （桂农科博2024058）
