中国瓜菜2026，Vol.39Issue(5)：38-47,10.DOI:10.16861/j.cnki.zggc.2025.0652
西瓜苗期耐低温综合评价体系构建及指标筛选
Construction of a comprehensive evaluation system and screening of indi-cators for low-temperature tolerance of watermelon seedlings
摘要
Abstract
This study aims to establish a comprehensive evaluation system for the low-temperature tolerance of watermel-on seedlings and screen key indicators.Through this system,the low-temperature tolerance of watermelon seedlings can be effectively and accurately evaluated,providing theoretical and technical support for the breeding of low-temperature tolerant varieties and early spring cultivation.Taking 16 watermelon materials with different genetic backgrounds as the research objects,the control was achieved by setting up low-temperature stress treatment at 4,6,8 and 10℃and normal temperature treatment at 25℃.The cold damage index was used as the screening index to determine the optimal stress conditions(treatment at 6℃for 3 days),and the change rate of four morphological indicators of seedlings,namely plant height,leaf length,leaf width and stem thickness,as well as five physiological indicators,namely superoxide dismutase(SOD)activity,peroxidase(CAT)activity,malondialdehyde(MDA)content,relative electrical conductivity and proline(Pro)content,under this low-temperature stress condition were measured.Principal component analysis(PCA)was adopt-ed to reduce the dimension and weight of the indicators.The low-temperature resistance of the test materials was compre-hensively evaluated and classified by combining the membership function method and cluster analysis.A prediction mod-el was established through regression analysis to screen the key indicators.Four principal components were extracted through principal component analysis,with a cumulative contribution rate of 83.411%.Through comprehensive evaluation(D value),16 materials were classified into 3 categories:WMN009,WMN016 and WMN003 are of strong low-tempera-ture resistance type;nine materials,including WMN010 and WMN002,are of medium low-temperature resistance type;four materials,namely WMN014 and WMN005,are of low-temperature sensitive type.Regression analysis further screened out five key indicators(CAT activity,SOD activity,plant height,leaf length,and relative electrical conductivi-ty),and established a reliable low-temperature tolerance prediction model(Y=1.012-0.108X3+0.017X4+0.195X5+0.749X6-0.072X9,R²=0.983).The comprehensive evaluation system and prediction model established in this study have important the value of practice in improving the accuracy and practicability of evaluating the low-temperature tolerance of watermelon seedlings.关键词
西瓜/低温胁迫/耐低温评价/预测模型Key words
Watermelon/Low-temperature stress/Low-temperature resistance evaluation/Predictive model分类
农业科技引用本文复制引用
海陶,史骏,张华峰,张蕾琛,胡紫蔚,应泉盛,何勇,王毓洪..西瓜苗期耐低温综合评价体系构建及指标筛选[J].中国瓜菜,2026,39(5):38-47,10.基金项目
宁波市重点研发计划暨"揭榜挂帅"项目(2023Z115) （2023Z115）
宁波市科技创新2025重大专项(2021Z132) （2021Z132）
国家西甜瓜产业技术体系(CARS-26) （CARS-26）
