中国油料作物学报2026，Vol.48Issue(2)：60-70,11.DOI:10.19802/j.issn.1007-9084.2025128
油菜苗期低温胁迫响应miRNA的鉴定及其靶基因预测分析
Identification of miRNAs response to low temperature stress and prediction of their target genes in rapeseed seedlings
摘要
Abstract
Low temperature stress severely influence the geographical distribution,growth and development,yield,and quality of rapeseed.In this study,to explore miRNAs response to low temperature and their target genes,elucidate the regulatory mechanism of resistance to low temperature stress in rapeseed,we used cold resistant rape-seed seedling leaves subjected to 6 hours of-3℃ stress and room temperature as material,constructed miRNA li-braries for high-throughput sequencing and bioinformatics analysis.A total of 76 known miRNAs and 200 novel miRNAs were identified,with 11 differentially expressed miRNAs,of which 5 were up-regulated and 6 down-regu-lated,and 618 corresponding target genes were predicted.GO analysis showed that the target genes were highly en-riched in regulation of COPI vesicle coat,intracellular protein transport,and transporter activity items in the three categories of cell component,biological process,and molecular function,respectively.KEGG analysis indicated that target genes have been enriched in endocytosis,RNA transport and amino sugar and nucleotide sugar metabolism.The qRT-PCR validation of differentially expressed miRNAs and their target genes showed that the five miRNAs exhibited negative regulatory relationships with their target genes.As a result,miRNAs in rapeseed can respond to low temperature stress by negatively regulating target genes,which can provide miRNAs and candidate target genes for the study of low-temperature stress mechanisms in rapeseed.关键词
油菜/miRNA/低温胁迫/靶基因/表达调控Key words
Brassica napus/miRNA/low temperature stress/target genes/expression regulation分类
农业科技引用本文复制引用
何俊平,王东国,朱家成,蔡东芳,曹金华,崔筱,陈培钰,张书芬,文雁成,刘奕孜,赵磊..油菜苗期低温胁迫响应miRNA的鉴定及其靶基因预测分析[J].中国油料作物学报,2026,48(2):60-70,11.基金项目
国家农业生物育种重大专项(2022ZD04010) （2022ZD04010）
河南省重点研发专项(221111110400) （221111110400）
河南省现代农业产业技术体系(HARS-22-06) （HARS-22-06）
国家油菜产业技术体系(CARS-12) （CARS-12）
河南省科技攻关(242102110320) （242102110320）
河南省科技研发计划联合基金(242301420119) （242301420119）
河南省农业科学院创新团队(2024TD20) （2024TD20）
河南省农业科学院基础性科研项目(2025JC08) （2025JC08）
