中国癌症防治杂志2026，Vol.18Issue(2)：200-209,10.DOI:10.3969/j.issn.1674-5671.2026.02.09
ST14在脑胶质瘤中的表达特征及其与预后和免疫微环境的相关性研究
Expression characteristics of ST14 in glioma and its association with prognosis and the immune microenvironment
摘要
Abstract
Objective To investigate the expression characteristics and prognostic correlation of transmembrane serine protease 14(ST14)in glioma,to analyze its association with the tumor immune microenvironment.Methods The expression pattern of ST14 in glioma and its associations with clinicopathological characteristics,prognosis,and immune infiltration were systematically analyzed based on public databases.Western blot was used to detect ST14 protein expression in brain tissues.After knocking down ST14 in the U251 cell line,transwell assays were used to detect cell migration and invasion ability,wound-healing assays were applied to assess cell migratory capacity,and cell counting kit-8(CCK-8)assays were conducted to quantify cell proliferation ability,so as to evaluate its effect on the malignant bio-logical behavior of glioma cells.Results ST14 was highly expressed in glioma,and its expression was associated with adverse molecular features,including isocitrate dehydrogenase(IDH)status and chromosome 1p/19q codeletion status(all P<0.05).The overall survival(OS)of the high ST14 expression group was significantly shortened(all P<0.001).Multivariate Cox regression analysis showed that ST14 remained independently associated with OS after adjustment for age,WHO grade,IDH status,and other factors(HR=1.246,95%CI:1.100-1.412,P<0.001).A nomogram incorporating ST14 and clinical characteristics could predict 1-,3-,and 5-year OS and achieve risk stratification.Immune analysis showed that high ST14 expression was associated with immune scores,altered immune cell infiltration patterns,and the expression of multiple immunoregulatory molecules(all P<0.001).Conclusions ST14 is aberrantly highly expressed in glioma.Knocking down ST14 can inhibit the proliferation,migration and invasion of U251 cells,and ST14 is associated with poor prognosis and immune microenvironment characteristics,suggesting that ST14 may serve as a candidate biomarker for prognostic evaluation and an auxiliary indica-tor for risk stratification in glioma.关键词
胶质瘤/ST14/丝氨酸蛋白酶/预后/免疫浸润Key words
Glioma/Transmembrane serine protease 14/Serine proteinase/Prognosis/Immune infiltration分类
医药卫生引用本文复制引用
苏田胜,李辉,闭剑斌,叶成林,严峻..ST14在脑胶质瘤中的表达特征及其与预后和免疫微环境的相关性研究[J].中国癌症防治杂志,2026,18(2):200-209,10.基金项目
广西自然科学基金项目(2023GXNSFDA026028 （）
2020GXNSFAA297154 （）
2018GXNSFAA281151) （）
国家自然科学基金项目(82260239 （）
82060225) （）
