中国真菌学杂志2026，Vol.21Issue(2)：147-153,7.DOI:10.3969/j.issn.1673-3827.2026.02.006
烟曲霉胶霉毒素通过mTOR/STAT3信号通路调控人肺泡Ⅱ型上皮细胞自噬的机制研究
Mechanism of gliotoxin from Aspergillus fumigatus regulating autophagy in human alveolar type II epithelial cells via the mTOR/STAT3 signaling pathway
摘要
Abstract
Objective Study on the effect and mechanism of gliotoxin-induced autophagy in human alveolar type Ⅱ epithelial cells via the mTOR/STAT3 pathway.Methods A549 cells were divided into control group,0.5 μmol/L,2 μmol/L,and 4 μmol/L gliotoxin-treated groups,and cultured for 24 hours.Cell growth status was observed using an inverted optical microscope.A549 cell viability was detected by CCK-8 assay.Intracellular ROS levels and apoptosis rate were measured by flow cytometry.The expression levels of autophagy-related proteins and mTOR/STAT3 signaling pathway-related proteins were analyzed by Western blot.Results Compared with the control group,the proliferative activity of A549 cells in the experimental group was significantly decreased.The 2 μmol/L and 4 μmol/L groups exhibited increased intracellular ROS levels and elevated apoptosis rates.MDC staining revealed significantly enhanced fluorescence intensity in the treated groups.The 2 μmol/L and 4 μmol/L groups showed increased expression of autophagy-related proteins Beclin-1 and LC3Ⅱ/LC3Ⅰ,while p62 expression was decreased.The protein expression levels of p-mTOR/mTOR and p-STAT3/STAT3 were significantly reduced.Conclusion Gliotoxin can inhibit proliferation,promote apoptosis,and induce autophagy in human alveolar type Ⅱ epithelial A549 cells,potentially through suppression of the mTOR/STAT3 signaling pathway activation.关键词
自噬/信号通路/胶霉毒素/肺泡Ⅱ型上皮细胞Key words
autophagy/signaling pathway/gliotoxin/type II alveolar epithelial cells引用本文复制引用
李莎莎,杨绍兵,焦婷婷,苏明,师志云,郭雅琪..烟曲霉胶霉毒素通过mTOR/STAT3信号通路调控人肺泡Ⅱ型上皮细胞自噬的机制研究[J].中国真菌学杂志,2026,21(2):147-153,7.基金项目
宁夏自然科学基金项目(2023AAC03531) （2023AAC03531）
