中国真菌学杂志2026，Vol.21Issue(2)：167-173,7.DOI:10.3969/j.issn.1673-3827.2026.02.009
食管上皮内瘤变与食管鳞状细胞癌真菌微生物特征分析
Fungal characteristics of esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma
摘要
Abstract
Objective To explore the alterations in esophageal fungal microecology during the development and progression of esophageal intraepithelial neoplasia(EIN)and esophageal squamous cell carcinoma(ESCC).Methods A case-control study was designed,including 8 patients with ESCC,5 patients with esophageal intraepithelial neoplasia(EIN),and 10 Individuals with a normal esophagus(NC)who underwent upper gastrointestinal endoscopy at the Second Hospital of Hebei Medical University from February 2023 to September 2023.Esophageal cell brush samples from each group were sequenced for the internal transcriptional spacer(ITS)and analyzed using bioinformatics method.Results Significant differences in esophageal fungal composition were observed among the three groups.Compared with NC,the microecological diversity of esophageal fungi was significantly reduced in both the EIN and ESCC groups,while the diversity between EIN and ESCC groups was similar,with no statistically significant difference.Inter-group comparisons revealed that compared to the NC group,the abundance of Yarrowia in the EIN and ESCC groups was significantly reduced in both disease groups(P<0.05).While the relative abundance of Chrysosporium was higher in the EIN group,the ESCC group exhibited an enrichment trend of Aspergillus.Despite the limited sample size,ROC analysis suggested show certain potential of Chrysosporium and Aspergillus in differentiating EIN from ESCC groups.The area under the ROC curve(AUC)was 70.0％(95％CI:38.2％-100.0％)for Chrysosporium and 73.8％(95％CI:49.5％-98.0％)for Aspergillus.Conclusion The composition of esophageal fungi is altered in patients with esophageal intraepithelial neoplasia(EIN)and esophageal squamous cell carcinoma(ESCC).Fungal genera such as Candida and Yarrowia may be involved in the carcinogenic process.Chrysosporium and Aspergillus exhibited distribution patterns associated with different disease stages in this study.These findings provide exploratory microecological evidence that may inform future early detection and treatment strategies for ESCC.关键词
食管鳞状细胞癌/食管上皮内瘤变/真菌微生态/生物标志物Key words
esophageal squamous cell carcinoma/esophageal intraepithelial neoplasia/fungal microecology/biomarker引用本文复制引用
宋怡康,郑琳,叶子渝,刘爱欣,马俊骥..食管上皮内瘤变与食管鳞状细胞癌真菌微生物特征分析[J].中国真菌学杂志,2026,21(2):167-173,7.基金项目
河北省2023年度医学科学研究课题(20230597) （20230597）
