中成药2026，Vol.48Issue(4)：1079-1086,8.DOI:10.3969/j.issn.1001-1528.2026.04.002
基于HPLC指纹图谱、化学计量学和含量测定评价苗药热淋清颗粒质量
Quality evaluation of Miao medicine Relinqing Granules based on HPLC fingerprints,chemometrics and content determination
摘要
Abstract
AIM To evaluate the quality of Miao medicine Relinqing Granules.METHODS The HPLC fingerprints were established,the analysis was performed on a 30℃thermostatic ShimNex CS C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of 0.2%phosphoric acid-methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 254 nm.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were performed,after which the contents of gallic acid,ellagic acid and quercitrin were determined.RESULTS There were 13 common peaks in the fingerprints for 47 batches of samples with the similarities of more than 0.871.Various batches of samples were clustered into two categories,three principal components demonstrated the accumulative variance contribution rate of 96.632%,and eight differential components were screened.Three constituents showed good linear relationships within their own ranges(R2≥0.999 7),whose average recoveries were 96.57%-101.85%with the RSDs of 0.70%-1.12%.CONCLUSION This simple,reliable and reproducible method can provide a scientific basis for the quality control and standard enhancement of Relinqing Granules.关键词
热淋清颗粒/质量评价/HPLC指纹图谱/聚类分析/主成分分析/正交偏最小二乘法-判别分析/含量测定Key words
Relinqing Granules/quality evaluation/HPLC fingerprints/cluster analysis/principal component analysis/orthogonal partial least squares discriminant analysis/content determination分类
医药卫生引用本文复制引用
陈莲,何雨其,赵鑫艳,唐靖雯,潘婷,杨伟鹏,唐策,张静,张艺..基于HPLC指纹图谱、化学计量学和含量测定评价苗药热淋清颗粒质量[J].中成药,2026,48(4):1079-1086,8.基金项目
国家重点研发计划"中医药现代化"重点专项(2023YFC3504402,2023YFC3504404,2023YFC3504405) （2023YFC3504402,2023YFC3504404,2023YFC3504405）
