中成药2026，Vol.48Issue(4)：1086-1092,7.DOI:10.3969/j.issn.1001-1528.2026.04.003
基于HPLC指纹图谱、化学模式识别和含量测定评价黄花倒水莲质量
Quality evaluation of Polygala fallax based on HPLC fingerprints,chemical pattern recognition and content determination
摘要
Abstract
AIM To evaluate the quality of Polygala fallax Hemsl.METHODS The HPLC fingerprints were established,the analysis was performed on a 35℃thermostatic Waters CORTECS T3 C18 column(2.1 mm×150 mm,1.6 μm),with the mobile phase comprising of acetonitrile-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 256 nm.Subsequently,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed,after which lancerin was used as an internal standard to calculate the relative correction factors of 7-O-methylmangiferin and polygalaxanthone Ⅲ,and the content determination was made by quantitative analysis of multi-components by single-marker(QAMS)method.RESULTS There were 5 common peaks in the fingerprints for 29 batches of medicinal materials with the similarities of more than 0.80(except for S2).Various batches of medicinal materials were clustered into two categories,7-O-methylmangiferin was quality differential marker.Three constituents showed good linear relationships within their own ranges(R2≥0.999 0),whose average recoveries were 95.34%-104.64%with the RSDs of 1.31%-2.30%.CONCLUSION This accurate and reliable method can provide evidence for the quality control of P.fallax.关键词
黄花倒水莲/质量评价/HPLC指纹图谱/聚类分析/主成分分析/正交偏最小二乘法-判别分析/含量测定/一测多评Key words
Polygala fallax Hemsl/quality evaluation/HPLC fingerprints/cluster analysis/principal component analysis/orthogonal partial least squares discriminant analysis/content determination/quantitative analysis of multi-components by single-marker(QAMS)分类
医药卫生引用本文复制引用
袁健童,梁祖兴,胡仁传,柴玲,黄勇波,谢晓婷,谷立勍,冯军,赖克道..基于HPLC指纹图谱、化学模式识别和含量测定评价黄花倒水莲质量[J].中成药,2026,48(4):1086-1092,7.基金项目
广西自然科学基金(2023GXNSFAA026089) （2023GXNSFAA026089）
广西壮族自治区中医药管理局项目(GXZYA20250013) （GXZYA20250013）
金秀瑶族自治县大健康产业发展中心项目(KJCYB-HX[2024]068) （KJCYB-HX[2024]068）
