中成药2026，Vol.48Issue(4)：1106-1114,9.DOI:10.3969/j.issn.1001-1528.2026.04.006
杜仲叶总黄酮提取工艺优化及其抗氧化活性评价
Optimization of extraction process for total flavonoids from Eucommiae folium leaves and evaluation of their anti-oxidant activity
摘要
Abstract
AIM To optimize the extraction process for total flavonoids from Eucommiae folium Oliv.leaves,and to evaluate their anti-oxidant activity.METHODS With ethanol concentration,liquid-solid ratio,soaking time and extraction time as influencing factors,the comprehensive score for total flavonoids,rutin,isoquercitrin,astragalin,phlorizin,quercetin,kaempferol contents and extract yield as evaluation index,the anti-oxidant activity was optimized by AHP-entropy weight TOPSIS method combined with Box-Behnken response surface method.Subsequently,the scavenging rates of DPPH·,ABTS+·free radicals were determined.RESULTS The optimal conditions were determined to be 60%for ethanol concentration,21∶1 for liquid-solid ratio,1.5 h for soaking time,2.0 h for extraction time,and two times for extraction frequency,the various constituent contents and extract yield were 79.671,0.131,1.127,0.035,2.143,0.855,0.157 mg/g and 37.534%.The IC50 values of total flavonoids on DPPH·,ABTS+·free radicals were 0.028,0.053 mg/mL,respectively.CONCLUSION This stable and reliable method can be used for the extraction of total flavonoids from E.folium leaves with strong anti-oxidant activity.关键词
杜仲叶/总黄酮/提取工艺/AHP-熵权TOPSIS法/Box-Behnken响应面法/抗氧化活性Key words
Eucommiae folium Oliv.leaves/total flavonoids/extraction process/AHP-entropy weight TOPSIS method/Box-Behnken response surface method/anti-oxidant activity分类
医药卫生引用本文复制引用
董小丽,谢兴文,张家旭,李婉玉,马趣环,石晓峰..杜仲叶总黄酮提取工艺优化及其抗氧化活性评价[J].中成药,2026,48(4):1106-1114,9.基金项目
甘肃省科技重大专项(22ZD6FA021-4) （22ZD6FA021-4）
第七届兰州市人才创新创业大赛项目(2024-02-07) （2024-02-07）
甘肃中医药大学研究生"创新创业基金"(2025CXCY-0-038) （2025CXCY-0-038）
