中成药2026，Vol.48Issue(4)：1123-1130,8.DOI:10.3969/j.issn.1001-1528.2026.04.008
基于NLRP3/Caspase-1/GSDMD信号通路探讨首荟通便胶囊对便秘小鼠结肠组织损伤的影响
Effects of Shouhui Tongbian Capsules on colon tissue injury in constipation mice based on NLRP3/Caspase-1/GSDMD signaling pathway
摘要
Abstract
AIM To investigate the effects of Shouhui Tongbian Capsules on colon tissue injury in constipation mice.METHODS C57BL/6J mice were randomly divided into control group,model group,mosapride group(3 mg/kg mosapride citrate dispersible tablets)and Shouhui low-dose and high-dose groups(150,300 mg/kg Shouhui Tongbian Capsules),with 12 mice in each group.Except for the control group,mice in other groups were given 10 mg/kg loperamide hydrochloride by gavage for 21 days to induce constipation models.After 7 days of modeling,they were given drugs by gavage continuously for 14 days.The excretion time of the first black stool in mice was observed,and the fecal moisture content and intestinal propulsion rate were measured.HE staining was used to observe the pathological morphology of colon tissue.Serum levels of IL-1β,IL-18 and TNF-α were detected by ELISA.Immunohistochemistry was used to detect the protein expressions of IL-18 and NLRP3,and the intestinal barrier protein expressions of Claudin-1 and Occludin in colon tissue.Western blot was used to detect the protein expressions of p38,p-p38,NF-κB,IL-1β,IL-6,IL-18,TNF-α,cleved-Caspase-1(C-Caspase-1)+Caspase-1,GSDMD+N-GSDMD,NLRP3 and ASC in colon.RT-qPCR was used to detect the mRNA expressions of p38,NF-κB,IL-1β,TNF-α,Caspase-1,GSDMD,NLRP3 and ASC in colon tissue.RESULTS Compared with the control group,the excretion time of the first black stool in the model group was prolonged(P＜0.05),and the fecal moisture content and intestinal propulsion rate were decreased(P＜0.05).Colon tissue showed inflammatory injury and barrier damage.Serum levels of IL-1β,IL-18 and TNF-α increased(P＜0.05).The protein expressions of p-p38/p38,NF-κB,IL-1β,IL-6,IL-18,TNF-α,C-Caspase-1/Caspase-1,N-GSDMD/GSDMD,NLRP3 and ASC in colon tissue were increased(P＜0.05),while the protein expressions of Claudin-1 and Occludin decreased(P＜0.05).The mRNA expressions of p38,NF-κB,IL-1β,TNF-α,Caspase-1,GSDMD,NLRP3 and ASC in colon tissue increased(P＜0.05).Compared with the model group,the excretion time of the first black stool in Shouhui high-dose group was shortened(P＜0.05),and the fecal moisture content and intestinal propulsion rate were increased(P＜0.05).Inflammatory injury and barrier damage of colon tissue recovered.Serum levels of IL-1β,IL-18 and TNF-α decreased(P＜0.05).The protein expressions of p-p38/p38,NF-κB,IL-1β,IL-6,IL-18,TNF-α,C-Caspase-1/Caspase-1,N-GSDMD/GSDMD,NLRP3 and ASC in colon tissue decreased(P＜0.05),while the protein expressions of Claudin-1 and Occludin increased(P＜0.05).The mRNA expressions of p38,NF-κB,IL-1β,TNF-α,Caspase-1,GSDMD,NLRP3 and ASC in colon tissue decreased(P＜0.05).CONCLUSION Shouhui Tongbian Capsules can improve the inflammatory injury and barrier damage of colon tissue in constipation mice,and its effect may be related to anti-inflammation and inhibition of activation of NLRP3/Caspase-1/GSDMD signaling pathway.关键词
首荟通便胶囊/便秘/肠道屏障/炎症因子/炎性损伤/NLRP3/Caspase-1/GSDMD信号通路Key words
Shouhui Tongbian Capsules/constipation/intestinal barrier/inflammatory factors/inflammatory injury/NLRP3/Caspase-1/GSDMD signaling pathway分类
医药卫生引用本文复制引用
张齐猛,梁勇,石宇,张洋,张虹玺..基于NLRP3/Caspase-1/GSDMD信号通路探讨首荟通便胶囊对便秘小鼠结肠组织损伤的影响[J].中成药,2026,48(4):1123-1130,8.基金项目
国家自然科学基金面上项目(82174371) （82174371）
辽宁科学技术计划项目(2022JH2/101300060) （2022JH2/101300060）
沈阳市科技计划项目(24-214-3-186,24-214-3-188) （24-214-3-186,24-214-3-188）
