中成药2026，Vol.48Issue(4)：1131-1137,7.DOI:10.3969/j.issn.1001-1528.2026.04.009
基于RIOK3-铁蛋白自噬轴探讨金叶败毒颗粒对脓毒症肺损伤大鼠的保护作用
Protective effects of Jinye Baidu Granules on sepsis-induced lung injury in rats based on the RIOK3-ferritin autophagy axis
摘要
Abstract
AIM To investigate the mechanism of Jinye Baidu Granules in improving lung injury in septic rats.METHODS Rats were randomly divided into blank group,sham operation group,model group,Jinye Baidu Granules group(Jinye group,1.28 g/kg),dexamethasone group(Dimi group,0.27 mg/kg)and Jinye Baidu Granules+dexamethasone group(Jinye+Dimi group,1.28 g/kg+0.27 mg/kg),with 6 rats in each group.Rat sepsis model was established by cecocentesis,and the drugs were given by gavage once immediately and 8 hours after modeling.Rats were killed 24 hours after modeling,lung tissue was taken and lung coefficient was calculated to evaluate pulmonary edema.The levels of total iron ion,Fe2+,Fe3+and malondialdehyde(MDA)in lung tissue were detected by colorimetry.The levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in lung tissue were detected by ELISA.HE staining was used to observe the pathological changes of lung tissue sections in each group.RT-qPCR method was used to detect the mRNA expressions of RIOK3 and NCOA4 in lung tissue.Western blot was used to detect the protein expressions of ferritin heavy chain(FTH1)and LC3B in lung tissue.RESULTS Compared with the sham operation group,the alveolar structure in the model group was obviously destroyed,the interstitial lung and alveoli were obviously edematous,the inflammatory cells were obviously infiltrated,the mRNA expressions of RIOK3 and NCOA4,the protein expression of LC3B,the levels of Fe2+,MDA,IL-6 and TNF-α,the lung coefficient were increased(P＜0.01),and the protein expression of FTH1 and the level of Fe3+were decreased(P＜0.01).Compared with the model group,the pathological injury of the right lung tissue of rats in each treatment group was improved to varying degrees.The mRNA expressions of RIOK3 and NCOA4,the protein expression of LC3B,the levels of Fe2+,MDA,IL-6 and TNF-α,and the lung coefficient were decreased(P＜0.05,P＜0.01),while the protein expression of FTH1 and the level of Fe3+were increased(P＜0.05,P＜0.01),among which Jinye+Dimi group had the best effect.CONCLUSION Jinye Baidu Granules can improve the inflammatory reaction,pulmonary edema and lung epithelial cell injury in septic rats by inhibiting the autophagy axis of RIOK3-ferritin,and its combined application with dexamethasone is more effective.关键词
金叶败毒颗粒/脓毒症/肺损伤/RIOK3-铁蛋白自噬轴Key words
Jinye Baidu Granules/sepsis/lung injury/RIOK3-ferritin autophagy axis分类
医药卫生引用本文复制引用
张雪玲,黄迪,梁明昊,许一飞,邱占军,陈宪海..基于RIOK3-铁蛋白自噬轴探讨金叶败毒颗粒对脓毒症肺损伤大鼠的保护作用[J].中成药,2026,48(4):1131-1137,7.基金项目
国家自然科学基金(82274320) （82274320）
山东省自然科学基金中医药联合基金培育项目(ZR2021LZY027) （ZR2021LZY027）
山东省自然科学基金委员会面上项(ZR2020MH392) （ZR2020MH392）
