中成药2026，Vol.48Issue(4)：1138-1148,11.DOI:10.3969/j.issn.1001-1528.2026.04.010
天芙止眩方通过调控α7nAChR表达对自发性高血压大鼠下丘脑室旁核小胶质细胞极化的影响
Effects of Tianfu Zhixuan Formula on microglial polarization in the hypothalamic paraventricular nucleus of spontaneously hypertensive rats via regulation of α7nAChR expression
摘要
Abstract
AIM To explore the effect of Tianfu Zhixuan Formula on microglia polarization in hypothalamic paraventricular nucleus(PVN)of spontaneously hypertensive rats(SHR).METHODS Forty SHR rats were randomly divided into model group,irbesartan group(13.5 mg/kg)and high,middle and low dose groups of Tianfu Zhixuan Formula(10.62,5.31 and 2.66 g/kg),with 8 rats in each group.All rats were administered intragastrically for 8 consecutive weeks.WKY rats were taken as normal group.The systolic blood pressure of tail artery of rats in each group was detected by noninvasive blood pressure meter.The levels of IL-1β and TNF-α in PVN were detected by ELISA.The co-expressions of CD86+/Iba-1+,CD163+/Iba-1+and α7nAChR+/Iba-1+in PVN were detected by immunofluorescence double labeling.RT-qPCR and Western blot were used to detect the mRNA and protein expressions of CD86,CD163 and α7nAChR in PVN.BV2 cells were induced by LPS,intervened by serum containing different concentrations of Tianfu Zhixuan Formula,and the cell viability was detected by MTT assay.The fluorescence intensity of α7nAChR/CD86 and α7nAChR/CD163 cells was detected by immunofluorescence double labeling.The protein expression of α7nAChR in cells was detected by Western blot.RESULTS Compared with the model group,the systolic blood pressure of the caudal artery,the levels of IL-1β and TNF-α in PVN,the expression of CD86+/Iba-1+,and the expression of CD86 mRNA and protein in irbesartan group and Tianfu Zhixuan Formula groups were all decreased(P＜0.05,P＜0.01),the expressions of CD163+/Iba-1+and α7nAChR+/Iba-1+,and the mRNA and protein expressions of CD163 and α7nAChR increased(P＜0.01).In BV2 cells induced by LPS,the serum containing Tianfu Zhixuan Formula could increase the expression of α7nAChR protein and the fluorescence intensity of α7nAChR and CD163(P＜0.05,P＜0.01),and decrease the fluorescence intensity of CD86(P＜0.05,P＜0.01).CONCLUSION Tianfu Zhixuan Formula may regulate the polarization of PVN microglia,reduce neuroinflammation and lower blood pressure in SHR rats by promoting α7nAChR expression.关键词
天芙止眩方/高血压/小胶质细胞/神经炎症/α7nAChRKey words
Tianfu Zhixuan Formula/hypertension/microglia/neuroinflammation/α7nAChR分类
医药卫生引用本文复制引用
江丽红,姚强,陆华冠,饶紫缇,谢新,王宇红,刘建军..天芙止眩方通过调控α7nAChR表达对自发性高血压大鼠下丘脑室旁核小胶质细胞极化的影响[J].中成药,2026,48(4):1138-1148,11.基金项目
湖南省自然科学基金项目(2024JJ8158) （2024JJ8158）
湖南中医药大学研究生创新课题(2024CX078,2024CX201) （2024CX078,2024CX201）
