浙江农业科学2026，Vol.67Issue(5)：1081-1087,7.DOI:10.16178/j.issn.0528-9017.20250914
浙樱粉1号高效制种及纯度鉴定技术
High-efficiency seed production and purity identification technology for Zheyingfen 1
摘要
Abstract
Zheyingfen 1 is a high-quality,parthenocarpy cherry tomato variety selected by Zhejiang Academy of Agricultural Sciences and widely cultivated in Zhejiang Province.The low seed production yield and mechanical mixing during the seed production process of Zheyingfen 1 have hindered the further promotion and application of this variety.Therefore,researching efficient seed production and purity identification technology for this variety,reducing seed production costs,and improving seed purity,is of great significance for further expanding the nationwide influence of independently selected tomato varieties of Zhejiang Province.This study systematically investigated the effects of different pruning methods(single plant double stem,double plant single stem,double plant double stem)and different fertilization treatments(T1,application of balanced water-soluble fertilizer;T2,partial replacement of balanced water-soluble fertilizer with high-potassium water-soluble fertilizer on the basis of T1 treatment;T3,additional application of calcium fertilizer on the basis of T2 treatment)on the seed production yield and related agronomic traits of Zheyingfen 1.Meanwhile,based on single nucleotide polymorphism(SNP)data from resequencing,SNP molecular markers based on kompetitive allele specific PCR(KASP)were developed to establish a high-throughput and low-cost rapid identification technology for seed purity of Zheyingfen 1.The results showed that the seed yield of the single plant double stem combined with T3 fertilization treatment was significantly(p＜0.05)higher than that of other treatments,with a seed yield of 11.76 kg per 667 m2.In addition,the three KASP-SNP molecular markers developed in this study can accurately identify the genetic purity of the hybrid Zheyingfen 1.关键词
樱桃番茄/浙樱粉1号/制种/纯度鉴定Key words
cherry tomato/Zheyingfen 1/seed production/purity identification分类
农业科技引用本文复制引用
刘晨旭,李志邈,刘丕杰,程远,姚祝平,朱丽娜,李素娟,张子鑫,万红建,王荣青,阮美颖,叶青静,周国治..浙樱粉1号高效制种及纯度鉴定技术[J].浙江农业科学,2026,67(5):1081-1087,7.基金项目
浙江省科技计划项目(2024C02003) （2024C02003）
宁波市科技特派员项目(2024S221) （2024S221）
金华市重大重点科技计划项目(2024-2-010) （2024-2-010）
