浙江农业科学2026，Vol.67Issue(5)：1097-1102,6.DOI:10.16178/j.issn.0528-9017.20250303
远缘杂交培育双单倍体丝瓜及其鉴定
Production and characterization of diploid Luffa by distant hybridization
摘要
Abstract
In this study,we developed diploid Luffa through distant hybridization induction technology,providing a theoretical basis for the cultivation and genetic improvement of excellent Luffa varieties.Luffa hybrid variety TL 01(2n=26)was utilized as the female parent,five different male parents of Cucurbitaceae were hybridized.The obtained hybrid offspring were subjected to ploidy identification and selfing to obtain the first generation.Simple sequence repeats(SSR)markers were used to identify whether they were diploids.The results showed that a total of 12 plump seeds were harvested from the five hybrid combinations.However,only watermelon pollen-induced harvested seeds were successfully regenerated from two plants,labeled T-1 and T-2.63 seeds were harvested from the selfing of T-1 plants.Using SSR markers to identify the first generation of selfing,it was found that six pairs of SSR markers had consistent band patterns among the first generation of selfing,indicating that the regenerated plant was diploid.The fertility of diploid Luffa has been restored and can be propagated using seeds.关键词
丝瓜/远缘杂交/双单倍体/分子标记Key words
Luffa/distant hybridization/diploid/molecular marker分类
农业科技引用本文复制引用
马夏彬,叶卢亮,端木李玲,陈卫民,郭勤卫,钱春桃,倪维晨..远缘杂交培育双单倍体丝瓜及其鉴定[J].浙江农业科学,2026,67(5):1097-1102,6.基金项目
常熟市农业科技项目(CN202406) （CN202406）
衢州市农业科技项目(2024K063) （2024K063）
