摘要
Abstract
Objective:To investigate the levels of serum DNA methylase 1(DNMT1),Mac-2 binding protein glycosylated isoform(M2BPGi)and their relationship with prognosis in patients with primary hepatocellular carcinoma(HCC).Methods:One hundred cases of HCC patients admitted to The 910th Hospital of the Chinese People's Liberation Army Joint Logistic Support Force and One hundred cases of healthy people with physical examination from May 2017 to July 2021 were selected as observation group and control group,respectively.The levels of DNMT1 and M2BPGi in the serum of the two groups were compared.The relationship between serum DNMT1 and M2BPGi levels and clinical pathological characteristics was analyzed using multiple regression analysis.After a 3-year follow-up,the survival rate of patients with different levels of serum DNMT1 and M2BPGi was compared.Results:The levels of serum DNMT1 and M2BPGi in the observation group were higher than those in the control group(P<0.05).Patients with AFP≥400 U/ml,HBsAg positive,clinical stage Ⅲ-Ⅳ,moderately and poorly differentiated,and lymph node metastasis had higher serum DNMT1 and M2BPGi levels than patients with AFP<400 U/ml,HBsAg negative,clinical stage Ⅰ-Ⅱ,well differentiated,and no lymph node metastasis(P<0.05).Clinical stage(biased regression coefficient=0.720/0.715),alpha-fetoprotein(biased regression coefficient=0.717/0.741),degree of differentiation(biased regression coefficient=0.764/0.819),hepatitis B surface antigen(biased regression coefficient=0.802/0.795),and lymph node metastasis(biased regression coefficient=0.804/0.802)were all the HCC patients with DNMT1 and M2BPGi(P<0.05).The 3-year overall survival rate of the high-level DNMT1 subgroup was40.82%,which was significantly lower than that of the low-level subgroup(63.83%)(P<0.05).The 3-year overall survival rate of the high-level subgroup of M2BPGi was 43.14%,which was significantly lower than that of the low-level subgroup's 62.22%(P<0.05).The 3-year survival rates of the DNMT1 high-level subgroup+M2BPGi high-level subgroup,DNMT1 low-level subgroup+M2BPGi high-level subgroup,DNMT1 high-level subgroup+M2BPGi low-level subgroup,and DNMT1 low-level subgroup+M2BPGi low-level subgroup were 40.82%,66.67%,74.47%,and 91.11%,respectively.The differences among the four groups were statistically significant(P<0.05).Conclusion:Serum DNMT1 and M2BPGi levels are high in HCC patients and correlate with patients'pathologic features,and detection of serum DNMT1 and M2BPGi can help to predict patients' prognostic survival.关键词
原发性肝细胞肝癌/DNA甲基化酶1/Mac-2结合蛋白糖基化异构体/病理特征/预后Key words
primary hepatocellular carcinoma/DNA methyltransferase1/Mac-2 binding protein glycosylation isoform/pathological characteristics/prognosis分类
医药卫生
