中药新药与临床药理2026，Vol.37Issue(5)：828-835,8.DOI:10.19378/j.issn.1003-9783.2026.05.006
伤科益肾壮骨丸调控Hippo-YAP/TAZ通路对膝骨关节炎大鼠软骨的影响
Effects of Shangke Yishen Zhuanggu Pills on Cartilage in Rats with Knee Osteoarthritis via Regulation of the Hippo-YAP/TAZ Pathway
摘要
Abstract
Objective To investigate the effects of Shangke Yishen Zhuanggu Pills on cartilage morphology in rats with knee osteoarthritis(KOA)by regulating the Hippo-YAP/TAZ pathway.Methods SD successfully modeled SD rats were divided into sham-operated group,model group,model+Shangke Yishen Zhuanggu Pills(0.5 g·kg-1)group,model+Shangke Yishen Zhuanggu Pills+si-NC group,and model+Shangke Yishen Zhuanggu Pills+si-LATS group,with 8 rats in each group.Morphological observation of rat knee articular cartilage was performed;Safranin O-Fast Green staining was used to observe the histomorphology of knee articular cartilage;TUNEL assay was conducted to detect chondrocyte apoptosis;immunohistochemical staining was performed to detect p-YAP1 expression;Western Blot was used to detect the expression of extracellular matrix and Hippo-YAP/TAZ pathway-related proteins.Results Compared with the sham-operated group,the knee articular cartilage in the model group appeared light yellow with a rough articular surface,ulcerated spots,and osteophytes;cartilage tissue showed pale Safranin O staining;the TUNEL-positive rate and MMP13 expression were significantly increased(P<0.05);while the expressions of Aggrecan(ACAN),Collagen type Ⅱ α1 chain(COL2A1),p-Large tumor suppressor kinase 1/2(p-LATS1/2),p-YAP1,and TAZ were significantly decreased(P<0.05).Compared with the model group,the knee articular cartilage in the model+Shangke Yishen Zhuanggu Pills group and the model+Shangke Yishen Zhuanggu Pills+si-NC group appeared milky white with a smooth articular surface,no cracks,and no osteophytes;cartilage tissue showed red staining;the TUNEL-positive rate and MMP13 expression were significantly decreased(P<0.05);while the expressions of ACAN,COL2A1,p-LATS1/2,p-YAP1,and TAZ were significantly increased(P<0.05);moreover,there was no statistically significant difference between the model+Shangke Yishen Zhuanggu Pills group and the model+Shangke Yishen Zhuanggu Pills+si-NC group(P>0.05).Compared with the model+Shangke Yishen Zhuanggu Pills+si-NC group,the knee articular cartilage in the model+Shangke Yishen Zhuanggu Pills+si-LATS group appeared light yellow with a rough,ulcerated articular surface;Safranin O staining was pale;the TUNEL-positive rate and MMP13 expression were significantly increased(P<0.05);while the expressions of ACAN,COL2A1,p-LATS1/2,p-YAP1,and TAZ were significantly decreased(P<0.05).Conclusion Shangke Yishen Zhuanggu Pills may alleviate knee articular cartilage degeneration and injury in rats with knee osteoarthritis by regulating the expression of key proteins in the Hippo-YAP/TAZ pathway,thereby maintaining normal cartilage morphology and extracellular matrix homeostasis.关键词
膝骨关节炎/软骨细胞/伤科益肾壮骨丸/Hippo-Yes相关蛋白/PDZ结合转录共激活因子通路/大鼠Key words
knee osteoarthritis/chondrocytes/Shangke Yishen Zhuanggu Pills/Hippo-YAP/TAZ pathway/rats分类
医药卫生引用本文复制引用
蔡青,盛浩,黄宇玮,裴轩烨,许敏,张朝驹,涂杨茂,马晓飞,朱元元,胡杰,吴磊磊,余文举..伤科益肾壮骨丸调控Hippo-YAP/TAZ通路对膝骨关节炎大鼠软骨的影响[J].中药新药与临床药理,2026,37(5):828-835,8.基金项目
湖北省自然科学基金项目(2024AFD348) （2024AFD348）
湖北省时珍人才工程科研项目(鄂卫函[2024]256号) （鄂卫函[2024]256号）
湖北省中医药管理局科研项目(ZY2025L015) （ZY2025L015）
荆州市科学技术局项目(2025HD131). （2025HD131）
