中药新药与临床药理2026，Vol.37Issue(5)：848-861,14.DOI:10.19378/j.issn.1003-9783.2026.05.008
基于网络药理学与实验验证探讨藏药五味甘露药浴组方治疗银屑病的作用机制
Exploring the Mechanism of Action of Tibetan Medicine Wuwei Ganlu Medicated Bath Formula in Trearting Psoriasis Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To investigate the key targets and pathways of the classic Tibetan medicine formula Wuwei Ganlu Medicated Bath in the treatment of psoriasis using network pharmacology and molecular docking techniques,and to validate the relevant findings through cellular experiments.Methods Essential oils from the constituent herbs of the Wuwei Ganlu Medicated Bath formula—Vkhan Pa,Mtshe Ldum,Shug Pa,Da Lis,and Vom Bu—were extracted using supercritical CO2 extraction and identified by gas chromatography-mass spectrometry(GC-MS).Key components and their targets were screened using databases such as SwissTargetPrediction.Psoriasis-related targets were obtained from databases including the Online Mendelian Inheritance in Man(OMIM).After identifying intersecting targets,a protein-protein interaction(PPI)network was constructed,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses.Molecular docking validation between core components and key targets was performed using software such as Autodock.Finally,in vitro cellular experiments were conducted to validate the predictions.A psoriasis inflammation model was established using lipopolysaccharide(LPS)-induced human adult low calcium high temperature(HaCaT)keratinocytes.Safe administration concentrations were determined using the Cell Counting Kit-8(CCK-8)assay.The regulatory effects of totarol,trans-nerolidol,lobetyolin acetate,and cryptomeridiol on the inflammatory factors interleukin-17(IL-17),interleukin-23(IL-23),interleukin-6(IL-6),and interferon-γ(IFN-γ)were detected by enzyme-linked immunosorbent assay(ELISA).Results From the 174 compounds identified by GC-MS,42 key components were selected based on a skin permeability coefficient(log Kp)≥-6 cm·s-1.A total of 109 intersecting drug-disease targets were obtained.PPI network and drug-component-disease-target network analyses identified 7 core targets,including signal transducer and activator of transcription 3(STAT3),Janus kinase 2(JAK2),Janus kinase 1(JAK1),B-cell lymphoma 2(BCL2),and epidermal growth factor receptor(EGFR).These interacted with core components such as totarol,trans-nerolidol,lobetyolin acetate,cryptomeridiol,and(4-phenyl)but-2-yl acetate,primarily involving pathways related to cell proliferation,apoptosis,immune regulation,and inflammation.Molecular docking indicated strong binding affinity of totarol with EGFR,JAK1,and BCL2.Cellular experiments confirmed that all components were non-cytotoxic at 20 µg·mL-1 and significantly inhibited the secretion of IL-17,IL-23,and IFN-γ(P＜0.01).Regarding IL-6 inhibition,lobetyolin acetate and cryptomeridiol showed significant effects(P＜0.01),and totarol also exhibited a notable effect(P＜0.05),confirming that these components can inhibit the expression of psoriasis-related inflammatory factors through multiple targets.Conclusion The Tibetan medicine Wuwei Ganlu Medicated Bath formula is a clinically common external treatment for psoriasis in Tibetan medicine due to its favorable efficacy.Through multi-dimensional"component-target-pathway"analysis and experimental validation,this study found that its core active components exert anti-inflammatory and immunomodulatory effects by targeting key targets such as STAT3 and JAK1,primarily via the JAK-STAT signaling pathway,inhibiting the expression of characteristic inflammatory factors including IL-17,IL-23,and IFN-γ.This elucidates the specific molecular mechanism underlying its therapeutic effect via the"Yellow Water(a pathological body fluid in Tibetan medicine)-skin-psoriasis"pathway from a modern pharmacological perspective,providing a basis for revealing the scientific connotation of the Wuwei Ganlu Medicated Bath formula in treating skin diseases.关键词
藏药/五味甘露药浴组方/银屑病/网络药理学/人永生化角质形成细胞/实验验证/分子对接/炎症因子Key words
Tibetan medicine/Wuwei Ganlu Medicated Bath formula/psoriasis/network pharmacology/human immortalized keratinocytes/experimental validation/molecular docking/inflammatory factors分类
医药卫生引用本文复制引用
白玛措,尕藏东智,文成当智,德吉卓玛,万代草,杨明宇,李智,热增才旦,杨本扎西..基于网络药理学与实验验证探讨藏药五味甘露药浴组方治疗银屑病的作用机制[J].中药新药与临床药理,2026,37(5):848-861,14.基金项目
国家自然科学基金项目(82460845) （82460845）
青海省科技计划项目(2022-ZJ-958Q) （2022-ZJ-958Q）
国家中医药管理局-全国名老中医药专家杨本扎西传承工作室项目. （）
