中药药理与临床2026，Vol.42Issue(4)：29-34,50,7.
二陈汤加味调控Hedgehog(HH)信号通路作用于慢性阻塞性肺疾病炎症的机制
Modified Erchentang Inhibits Inflammation in Chronic Obstructive Pulmonary Disease via Hedgehog(HH)Signaling Pathway
摘要
Abstract
Objective:To explore the therapeutic mechanism of modified Erchentang(二陈汤)on inflammation in chronic obstructive pulmonary disease(COPD)via the Hedgehog(HH)signaling pathway.Methods:The rat model of COPD was established by cigarette smoke exposure combined with intratracheal instillation of lipopolysaccharide(LPS).The serum levels of interleukin-4(IL-4)and interleukin-13(IL-13)were measured by enzyme-linked immunosorbent assay.The mRNA levels of sonic hedgehog(Shh),patched 1(Ptch1),and glioma-associated oncogene homolog 1(Gli1)were determined by real-time PCR.The protein levels of HH signaling pathway-related factors GLI1,SHH,and PTCH1 in cells were detected by immunohistochemistry.Hematoxylin-eosin staining was conducted to reveal the morpho-logical changes of the rat lung tissue.Results:Compared with the normal control group,the model control group showed reduced forced expiratory volume in the first second(FEV1),forced vital capacity(FVC),and FEV1/FVC ratio(P<0.01),elevated serum levels of IL-4 and IL-13(P<0.01),and up-regulated mRNA and protein levels of Shh,Ptch1,and Gli1 in the lung tissue(P<0.01).Compared with the model control group,the cyclopamine group and the modified Erchentang(20,10 g/kg)groups showed increases in FEV1/FVC ratio,FEV1,and FVC(P<0.05 or P<0.01).The modified Erchentang(5,10,and 20 g/kg)groups and the cyclopamine group showed declined serum levels of IL-4 and IL-13(P<0.01)and down-regulated mRNA and protein levels of Shh,Ptch1,and Gli1 in the lung tissue(P<0.05 or P<0.01).Conclusion:The HH signaling pathway was significantly activated in the rat model of COPD induced by ciga-rette smoke exposure combined with intratracheal instillation of LPS,with up-regulated expression of HH signaling path-way-related factors.Modified Erchentang may inhibit the activation of the downstream regulatory factor GLI1 of the HH signaling pathway by down-regulating the mRNA levels of Shh,Ptch1,and Gli1,thereby reducing the release of IL-4 and IL-13 and inhibiting the inflammation in COPD.关键词
二陈汤/慢性阻塞性肺疾病/刺猬信号通路/炎症/跨膜蛋白受体1/神经胶质瘤相关癌基因同源物1(GLI1)Key words
Erchentang/Chronic obstructive pulmonary disease/Hedgehog signaling pathway/Inflammation/Patched 1(PTCH1)/Glioma-associated oncogene homolog 1(GLI1)引用本文复制引用
任鹏飞,季书,刘欢欢,尚立芝,赵晗方,王栋梁,尚皓梵,王琦..二陈汤加味调控Hedgehog(HH)信号通路作用于慢性阻塞性肺疾病炎症的机制[J].中药药理与临床,2026,42(4):29-34,50,7.基金项目
国家自然科学基金面上项目(编号:81573881) （编号:81573881）
河南省科技攻关项目(编号:182102311163) （编号:182102311163）
河南省高等学校重点科研项目(编号:25B360003) （编号:25B360003）
郑州西亚斯学院2024年度科研资助项(编号:2024XKD081,2024XKD094) （编号:2024XKD081,2024XKD094）
