中医药信息2026，Vol.43Issue(5)：33-38,54,7.DOI:10.19656/j.cnki.1002-2406.20260505
黄芪甲苷对间充质干细胞分泌外泌体炎性miRNAs和炎性因子的影响
Effects of Astragaloside Ⅳ on Exosomal Inflammatory miRNAs and Inflammatory Factors Secreted by Mesenchymal Stem Cells
摘要
Abstract
Objective To explore the effect of astragaloside Ⅳ(AS-Ⅳ)on exosomal inflammatory miRNAs and inflammatory factors secreted by mesenchymal stem cells(MSCs).Methods MSCs were isolated and identified from umbilical cord blood of healthy newborns and randomly divided into experimental and control groups.The experimental group was intervened with 300 mg/L AS-Ⅳ,while the control group was treated with equal volume of PBS solution.The exosomal MSC-Exos secreted by MSCs was extracted using ultracentrifugation combined with ultrafiltration after 24 hours of culture.Transmission electron microscopy was used to observe the morphology of MSC-Exos;the concentration of MSC-Exos was measured by BCA method;the expression of inflammation-related miRNAs was detected by RT-qPCR,and the levels of IL-1β,IL-6,IL-10,M-CSF and MCP-1 was detected by ELISA.Results After 24 hours of culture,the morphology of MSC-Exos was a vesicle-like structure with intact envelope under electron microscopy.The concentration of MSC-Exos in the experimental group was(1.215±0.018)μg/μL,and the concentration of MSC-Exos in the control group was(0.895±0.017)μg/μL,with a statistically significant difference(P<0.01).Compared with the control group,the experimental group exhibited a significant upregulation of miR-21-3p,miR-125b-5p,and miR-126-3p,the expression of miR-31-5p,miR-126-5p,and miR-221 was downregulated,with a statistically significant difference(P<0.05).No statistically significant differences were observed in miR-145 and miR-146a-5p expression between the two groups(P>0.05).Compared with the control group,the experimental group secreted MCP-1 and M-CSF levels decreased,and secreted IL-6 and IL-10 levels increased,with statistically significant differences(P<0.05),while the difference in IL-1β secretion between the two groups was not statistically significant(P>0.05).Conclusion AS-Ⅳ modulates inflammatory responses by promoting MSCs-derived exosomal secretion of inflammation-related miRNAs and suppressing pro-inflammatory factor release.关键词
黄芪甲苷/间充质干细胞/外泌体/miRNAs/炎性因子Key words
Astragaloside Ⅳ/Mesenchymal stem cells/Exosomes/miRNAs/Inflammatory factors引用本文复制引用
李思颖,邹晓玲,贺有缘,刘向男,薛冰倩,乔绍叶,王莹,宾马赢,罗佳,熊武..黄芪甲苷对间充质干细胞分泌外泌体炎性miRNAs和炎性因子的影响[J].中医药信息,2026,43(5):33-38,54,7.基金项目
湖南省自然科学基金医卫联合项目(2025JJ80930) （2025JJ80930）
长沙市自然科学基金项目(kq2502258) （kq2502258）
湖南省教育厅优秀青年项目(24B0368) （24B0368）
