茶叶学报2026，Vol.67Issue(2)：21-26,6.DOI:10.20045/j.cnki.issn.2096-0220.2026.02.003
外源激素对'中茶108'插穗生根、氧化酶活性及内源激素的影响
Rooting,Enzyme Activities,and Endogenous Hormones of Zhongcha 108 Cuttings Affected by Plant Regulator Application
摘要
Abstract
[Objective]Rooting,enzyme activities,and endogenous hormones of Zhongcha 108 stem cuttings were studied to determine the applicability of root growth promotors for plant propagating.[Methods]Different types of growth regulators in varied concentrations were applied on Zhongcha 108 stem cuttings to determine the effects on rooting and plant physiology.Activities of indole acetic acid oxidase(IAAO),peroxidase(POD),and polyphenol oxidase(PPO)of the plantlets were detected by spectrophotometry,and contents of endogenous indole acetic acid(IAA),abscisic acid(ABA),and zeatin riboside(ZR)by ELISA.[Results]Application of exogenous hormones,1-naphthaleneacetic acid(NAA)and indole-3-butyric acid(IBA),promoted rooting of the cuttings.Although increasing NAA concentration brought about greater root growth,the application at 150 mg·L-1 rendered the highest growth rate of 89.82%and fresh root weight of 0.18 g,as well as 8.26 and averaging 3.78 longest roots per cutting.The applied regulators raised the content of IAA and ABA with a declined activity of IAAO in the tissue which stimulated root growth.[Conclusion]By applying 150 mg·L-1 NAA on Zhongcha 108 stem cuttings,rooting of the plantlets was significantly enhanced to facilitate the propagation program.关键词
茶树/扦插/NAA/IBA/内源激素/氧化酶活性Key words
Tea plant(Camellia sinensis)/stem cutting/NAA/IBA/endogenous hormones/oxidase activity分类
农业科技引用本文复制引用
胡双玲,周洋,陈芳,陈清华,康绍玲,张强,梁金波,罗鸿,向俊,黄忠浩..外源激素对'中茶108'插穗生根、氧化酶活性及内源激素的影响[J].茶叶学报,2026,67(2):21-26,6.基金项目
湖北省自然科学基金项目(2023AFD065) （2023AFD065）
恩施州农科院青年创新基金项目(2023-005). （2023-005）
