动物营养学报2026，Vol.38Issue(5)：3403-3416,14.DOI:10.12418/CJAN2026.272
饲粮中油脂通过成纤维细胞生长因子23-Klotho-维生素D轴影响蛋鸡的蛋品质
Dietary Oils Affects Egg Quality of Laying Hens via Fibroblast Growth Factor 23-Klotho-Vitamin D Axis
摘要
Abstract
This study aimed to investigate the effects of dietary oil supplementation on egg quality of laying hens and to elucidate the underlying mechanism from the perspective of oil-mediated regulation of vitamin D metabolism and the fibroblast growth factor 23(FGF23)-Klotho axis.A total of 144 Roman pink laying hens aged 65 weeks,with no significant difference in laying rate among groups(P＞0.05),were randomly divided into 4 groups,each consisting of 6 replicates with 6 hens per replicate.Group A(control group)was fed a basal diet without oil supplementation,while the other three groups(experimental groups)were fed experi-mental diets supplemented with 2.0%soybean oil(group B),1.4%soybean oil+0.6%lard(group C)or 2.0%composite oil(0.5%soybean oil+0.5%corn oil+0.5%palm oil+0.5%coconut oil)(group D).The pre-feeding period lasted for 7 days,followed by a formal experimental period of 45 days.The results showed as follows:1)there were no significant differences among groups in average daily feed intake,average daily egg mass,feed-to-egg ratio,laying rate,soft-shell egg rate and breaking egg rate(P＞0.05).Compared with the control group,albumen height and Haugh unit were significantly increased in the three experimental groups(P＜0.05),while eggshell strength showed a numerical increase(P＞0.05).2)Compared with the control group,the contents of linoleic acid(C18∶2n-6),α-linolenic acid(C18∶3n-3),arachidonic acid(C20∶4),docosahexaenoic acid(C22∶6n-3,DHA),n-6 polyunsaturated fatty acids(PUFA),n-3 PUFA and PUFA in egg yolk in the three experimental groups were significantly increased(P＜0.05),whereas the contents of palmitoleic acid(C16∶1),trans-oleic acid(C18∶1 trans),cis-oleic acid(C18∶1 cis)and monounsaturated fatty acids(MUFA)were significantly decreased(P＜0.05).The n-6/n-3 PUFA ratio in the control group was 20.0,and compared with the control group,this ratio was significantly reduced by 4.7,4.6 and 1.7 in groups B,C and D,respectively(P＜0.05).Meanwhile,compared with the control group,saturated fatty acid(SFA)content in egg yolk was decreased by 1.20%in group B(P＞0.05),1.98%in group C(P＜0.05),and 1.21%in group D(P＞0.05),whereas unsaturated fatty acid(UFA)content was increased by 1.38%in group B(P＞0.05),1.89%in group C(P＜0.05),and 1.10%in group D(P＞0.05).3)Compared with the control group,serum contents of 1,25-dihydroxyvitamin D3[1,25-(OH)2 D3],FGF23,Klotho and calcium were significantly increased in the three experimental groups(P＜0.05);in addition,serum phosphorus content was significantly increased in group C(P＜0.05).4)The relative mRNA expression levels of cytochrome P450 family 27 subfamily B member 1(CYP27B1)in the kidney,as well as membrane vitamin D receptor(mVDR),nuclear vitamin D receptor(nVDR)and solute carrier family 34 member 2(SLC34A2)in the duo-denum,were significantly higher in the three experimental groups than in the control group(P＜0.05).In con-clusion,dietary supplementation with different oils(2.0%soybean oil,1.4%soybean oil+0.6%lard,or 2.0%composite oil)in laying hen diets can improve calcium and phosphorus metabolism and the activation of vita-min D by upregulating the expression of CYP27B1 in the kidney and mVDR,nVDR and SLC34A2 in the duo-denum,thereby enhancing egg quality.Additionally,oil supplementation increases egg yolk PUFA content while decreasing SFA content,thereby optimizing egg yolk fatty acid composition and the n-6/n-3 PUFA ratio.[Chinese Journal of Animal Nutrition,2026,38(5):3403-3416]关键词
油脂/维生素D/FGF23-Klotho-维生素D轴/蛋黄脂肪酸组成Key words
oils/vitamin D/FGF23-Klotho-vitamin D axis/egg yolk fatty acid composition分类
农业科技引用本文复制引用
李逍遥,谢越盛,刘福,袁顺华,赵玉蓉,曹满湖..饲粮中油脂通过成纤维细胞生长因子23-Klotho-维生素D轴影响蛋鸡的蛋品质[J].动物营养学报,2026,38(5):3403-3416,14.基金项目
国家十四五重点研发计划(2021YFD1300204) （2021YFD1300204）
