福建农业学报2026，Vol.41Issue(2)：137-147,11.DOI:10.19303/j.issn.1008-0384.2026.02.001
盐肤木LBD基因家族鉴定及热胁迫响应表达分析
Identification and Expressions under Heat Stress of LBD Family in Rhus chinensis
摘要
Abstract
[Objective]Genome-wide identification,biological functions,and expressions under heat stress of the LBD family in Rhus chinensis Mill were studied.[Methods]Based on the whole genome of R.chinensis,bioinformatics methods were applied to identify the LBD family members and analyze their physicochemical properties,conserved motifs,chromosomal localization,phylogeny,and collinearity.Transcriptomes were used to determine the expressions of RcLBDs in tissues and predict the key cis-acting elements on the promoters from online database.Subcellular localizations of the genes were examined by GFP fused expression in tobacco leaves,and expressions under heat stress by qRT-PCR.[Results]Thirty-two members in the LBD family were identified from the whole genome of R.chinensis and classified into 5 subfamilies based on the genetic distance.Structurally,they were mainly GAS glycine conserved domains,CX2CX6CX3C zinc fingers,and leucine zippers,and expressed significantly higher in the non-embryonic callus tissues than in the others.The promoter regions of RcLBDs were rich in various cis-acting components that responded to abiotic stress,hormones,growth,and development.Based on the analysis of promoter cis-acting components,3 members were selected that included the pan-localized RcLBD16 and the nuclear-localized RcLBD19 and RcLBD23 with expressions significantly upregulated by heat stress.[Conclusion]Of the 32 RcLBDs identified in R.chinensis,RcLBD16,RcLBD19,and RcLBD23 were presumably associated with the response to heat stress.关键词
盐肤木/LBD基因家族/生物信息学分析/亚细胞定位/热胁迫Key words
Rhus chinensis Mill./LBD gene family/bioinformatics analysis/subcellular localization/heat stress分类
农业科技引用本文复制引用
朱亚静,胡珂,巴广,梁宏伟,陈发菊,刘文..盐肤木LBD基因家族鉴定及热胁迫响应表达分析[J].福建农业学报,2026,41(2):137-147,11.基金项目
国家自然科学基金项目(32370393) （32370393）
